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Tetracycline hydrochloride

Name: Tetracycline hydrochloride
Brand: TargetMol
SKU: T0912
Price: 41 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0912Cas No. 64-75-5

Alias Tetracycline HCl, NCI-c55561

Tetracycline hydrochloride (NCI-c55561) is the hydrochloride salt of the tetracycline, a broad-spectrum naphthacene antibiotic produced semisynthetically from chlortetracycline, an antibiotic isolated from the bacterium Streptomyces aureofaciens. In bacteria, tetracycline blocks binding of aminoacyl-tRNA to the mRNA-ribosome complex, thereby inhibiting protein synthesis and bacterial cell growth. Because naturally, fluorescing tetracycline binds to a newly formed bone at the bone/osteoid interface, tetracycline-labeling of bone and fluorescence microscopy may be used to perform bone histomorphometry.

Tetracycline hydrochloride

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Purity: 99.64%

Catalog No. T0912Alias Tetracycline HCl, NCI-c55561Cas No. 64-75-5

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$41	In Stock	In Stock
1 g	$59	-	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.64%

Appearance:Solid

Color:Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Tetracycline hydrochloride (NCI-c55561) is the hydrochloride salt of the tetracycline, a broad-spectrum naphthacene antibiotic produced semisynthetically from chlortetracycline, an antibiotic isolated from the bacterium Streptomyces aureofaciens. In bacteria, tetracycline blocks binding of aminoacyl-tRNA to the mRNA-ribosome complex, thereby inhibiting protein synthesis and bacterial cell growth. Because naturally, fluorescing tetracycline binds to a newly formed bone at the bone/osteoid interface, tetracycline-labeling of bone and fluorescence microscopy may be used to perform bone histomorphometry.
In vitro	Tetracyclines are versatile broad-spectrum antibiotics effective against a vast array of organisms, including gram-positive and gram-negative bacteria, as well as atypical pathogens like chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites. Their mechanism of action involves blocking bacterial protein synthesis by hindering the binding of aminoacyl-tRNA to the bacterial ribosome. Specifically, in gram-negative enteric bacteria, tetracyclines enter through OmpF and OmpC porin channels as positively charged coordination complexes, likely involving magnesium ([1]).
In vivo	Tetracyclines are utilized for managing infections in various livestock, including poultry, cattle, sheep, and swine, and in aquaculture species such as salmon, catfish, and lobsters[2]. In specific scenarios, such as treating large populations of commercially farmed poultry, these antibiotics may be administered directly through feed, water, or aerosols. Additionally, tetracyclines may serve to promote or enhance growth.
Synonyms	Tetracycline HCl, NCI-c55561

Chemical Properties

Molecular Weight	480.90
Formula	C₂₂H₂₄N₂O₈·HCl
Cas No.	64-75-5
Smiles	C[C@@]1([C@H]2C[C@H]3[C@@H](C(=C(C(=O)[C@]3(C(=C2C(=O)c2c1cccc2O)O)O)C(=O)N)O)N(C)C)O.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 126 mg/mL (262.01 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: 88 mg/mL (182.99 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (8.32 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0794 mL	10.3972 mL	20.7943 mL	103.9717 mL
5 mM	0.4159 mL	2.0794 mL	4.1589 mL	20.7943 mL
10 mM	0.2079 mL	1.0397 mL	2.0794 mL	10.3972 mL
20 mM	0.1040 mL	0.5199 mL	1.0397 mL	5.1986 mL
50 mM	0.0416 mL	0.2079 mL	0.4159 mL	2.0794 mL
100 mM	0.0208 mL	0.1040 mL	0.2079 mL	1.0397 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc