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SCH529074

Name: SCH529074
Brand: TargetMol
SKU: T37042
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T37042Cas No. 922150-11-6

Alias SCH 529074

SCH529074 is a selective activator of p53 (Ki = 1 μM) and can be used in studies about non-small-cell lung carcinoma. SCH529074 restores mutant p53 function and interrupts HDM2-mediated ubiquitination of wild Type p53.

SCH529074

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Purity: 99.57%

Catalog No. T37042Alias SCH 529074Cas No. 922150-11-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	-	In Stock
2 mg	$38	-	In Stock
5 mg	$58	-	In Stock
10 mg	$106	-	In Stock
25 mg	$232	-	In Stock
50 mg	$345	-	In Stock
100 mg	$493	-	In Stock
1 mL x 10 mM (in DMSO)	$69	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.57%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	SCH529074 is a selective activator of p53 (Ki = 1 μM) and can be used in studies about non-small-cell lung carcinoma. SCH529074 restores mutant p53 function and interrupts HDM2-mediated ubiquitination of wild Type p53.
Targets&IC50	p53 DBD:1-2 μM (Ki)
In vitro	SCH529074 (2-4 µM) reduces cell viability and decreases to 20-25% in p53 mutant cells including H157, H1975, and H322, and to 68% in the p53 WT cell line A549 at 4 µM. SCH 529074 (2-4 µM) induces NSCLC cells including H157, A549, HCT116, and HCT116 p53-/- arrested at the G0/G1 phase (59%; 72%; 66%; and 57%)[2].
In vivo	In female nude mice, 5–7 weeks of age, received subcutaneous inoculation of DLD-1 human colorectal carcinoma cells, SCH529074 (orally; 30 and 50 mg/kg) reduces 79 and 43% of tumor growth with the plasma exposure of 0.26–0.55 μm at 30 mg/kg and 0.39-0.79 μm at 50 mg/kg[1].
Synonyms	SCH 529074

Chemical Properties

Molecular Weight	563.56
Formula	C₃₁H₃₆Cl₂N₆
Cas No.	922150-11-6
Smiles	CN(C)CCCNc1nc(CN2CCN(CC2)C(c2ccc(Cl)cc2)c2ccc(Cl)cc2)nc2ccccc12

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 33 mg/mL (58.56 mM), Sonication is recommended.

DMF: 33 mg/mL (58.56 mM), Sonication is recommended.

DMSO: 22.5 mg/mL (39.92 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (1.77 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol/DMF

	1mg	5mg	10mg	50mg
1 mM	1.7744 mL	8.8722 mL	17.7443 mL	88.7217 mL
5 mM	0.3549 mL	1.7744 mL	3.5489 mL	17.7443 mL
10 mM	0.1774 mL	0.8872 mL	1.7744 mL	8.8722 mL
20 mM	0.0887 mL	0.4436 mL	0.8872 mL	4.4361 mL

Ethanol/DMF

	1mg	5mg	10mg	50mg
50 mM	0.0355 mL	0.1774 mL	0.3549 mL	1.7744 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc