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PIT

Name: PIT
Brand: TargetMol
SKU: T23164
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T23164Cas No. 56583-49-4

Alias 2,2'-Pyridylisatogen tosylate

PIT (2,2'-Pyridylisatogen tosylate) is a specific, non-competitive antagonist of the Purinergic receptor P2Y1, exhibiting an IC50 of 0.14 μM for human P2Y1. It does not affect nucleotide binding and is useful in studies related to chronic bronchitis and asthma.

PIT

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Purity: 98.63%

Catalog No. T23164Alias 2,2'-Pyridylisatogen tosylateCas No. 56583-49-4

Pack Size	Price	Availability
5 mg	$34	In Stock
10 mg	$58	In Stock
25 mg	$118	In Stock
50 mg	$198	In Stock

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Purity:98.63%

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Product Introduction

Bioactivity

Description	PIT (2,2'-Pyridylisatogen tosylate) is a specific, non-competitive antagonist of the Purinergic receptor P2Y1, exhibiting an IC50 of 0.14 μM for human P2Y1. It does not affect nucleotide binding and is useful in studies related to chronic bronchitis and asthma.
Targets&IC50	P2Y1:0.14 μM
In vitro	PIT (100 nM-10 μM) diminishes human P2Y1 receptor signaling in a non-competitive and dose-dependent manner and dose-dependently blocks the agonist activity of 2-MeSADP[1]. PIT (0.1-3 μM) increases ATP-responses 2-5 fold. PIT (3-100 μM) inhibits ATP-mediated inward current (IC50 = 13.2 μM). PIT shows affinity to adenosine receptor (pKi = 5.3)[2].
In vivo	In mice with S-bromo-willardiine injection induced tonic and tonicoclonic seizures, intraperitoneal injection of PIT (10 mg/kg) prevents the white matter and the cortical plate lesions against the insult[3].
Synonyms	2,2'-Pyridylisatogen tosylate

Chemical Properties

Molecular Weight	396.42
Formula	C₂₀H₁₆N₂O₅S
Cas No.	56583-49-4
Smiles	Cc1ccc(cc1)S(O)(=O)=O.[O-][N+]1=C(C(=O)c2ccccc12)c1ccccn1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 75.0 mg/mL (189.2 mM), Sonication and heating to 70℃ are recommended.

Ethanol: < 5.95 mg/mL, Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5226 mL	12.6129 mL	25.2258 mL	126.1289 mL
5 mM	0.5045 mL	2.5226 mL	5.0452 mL	25.2258 mL
10 mM	0.2523 mL	1.2613 mL	2.5226 mL	12.6129 mL
20 mM	0.1261 mL	0.6306 mL	1.2613 mL	6.3064 mL
50 mM	0.0505 mL	0.2523 mL	0.5045 mL	2.5226 mL
100 mM	0.0252 mL	0.1261 mL	0.2523 mL	1.2613 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc