Shopping Cart

Your shopping cart is currently empty

PIT

Name: PIT
Brand: TargetMol
SKU: T23164
Price: 97 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T23164Cas No. 56583-49-4

Alias 2,2'-Pyridylisatogen tosylate

PIT (2,2'-Pyridylisatogen tosylate) is a specific, non-competitive antagonist of the Purinergic receptor P2Y1, exhibiting an IC50 of 0.14 μM for human P2Y1. It does not affect nucleotide binding and is useful in studies related to chronic bronchitis and asthma.

PIT

🥰Excellent

Purity: 98.63%

Catalog No. T23164Alias 2,2'-Pyridylisatogen tosylateCas No. 56583-49-4

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$97	In Stock	In Stock
10 mg	$168	In Stock	In Stock
25 mg	$354	In Stock	In Stock
50 mg	$568	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:98.63%

Appearance:Solid

Color:Red

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	PIT (2,2'-Pyridylisatogen tosylate) is a specific, non-competitive antagonist of the Purinergic receptor P2Y1, exhibiting an IC50 of 0.14 μM for human P2Y1. It does not affect nucleotide binding and is useful in studies related to chronic bronchitis and asthma.
Targets&IC50	P2Y1:0.14 μM
In vitro	PIT (100 nM-10 μM) diminishes human P2Y1 receptor signaling in a non-competitive and dose-dependent manner and dose-dependently blocks the agonist activity of 2-MeSADP[1]. PIT (0.1-3 μM) increases ATP-responses 2-5 fold. PIT (3-100 μM) inhibits ATP-mediated inward current (IC50 = 13.2 μM). PIT shows affinity to adenosine receptor (pKi = 5.3)[2].
In vivo	In mice with S-bromo-willardiine injection induced tonic and tonicoclonic seizures, intraperitoneal injection of PIT (10 mg/kg) prevents the white matter and the cortical plate lesions against the insult[3].
Synonyms	2,2'-Pyridylisatogen tosylate

Chemical Properties

Molecular Weight	396.42
Formula	C₂₀H₁₆N₂O₅S
Cas No.	56583-49-4
Smiles	Cc1ccc(cc1)S(O)(=O)=O.[O-][N+]1=C(C(=O)c2ccccc12)c1ccccn1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 75 mg/mL (189.19 mM), Sonication and heating to 70℃ are recommended.

Ethanol: < 5.95 mg/mL, Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 3.3 mg/mL (8.32 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5226 mL	12.6129 mL	25.2258 mL	126.1289 mL
5 mM	0.5045 mL	2.5226 mL	5.0452 mL	25.2258 mL
10 mM	0.2523 mL	1.2613 mL	2.5226 mL	12.6129 mL
20 mM	0.1261 mL	0.6306 mL	1.2613 mL	6.3064 mL
50 mM	0.0505 mL	0.2523 mL	0.5045 mL	2.5226 mL
100 mM	0.0252 mL	0.1261 mL	0.2523 mL	1.2613 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc