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Oxythiamine

Name: Oxythiamine
Brand: TargetMol
SKU: T12354
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12354Cas No. 136-16-3

Alias Hydroxythiamin

Oxythiamine (Hydroxythiamin) is an antivitamin of thiamine and a transketolase (TK) inhibitor. Oxythiamine inhibits cancer cell proliferation and induces cell cycle arrest. Oxythiamine has potential anticancer activity, inhibits non-oxidative synthesis of ribose, and induces apoptosis.

Oxythiamine

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Purity: 99.51%

Catalog No. T12354Alias HydroxythiaminCas No. 136-16-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 mg	$29	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.51%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Oxythiamine (Hydroxythiamin) is an antivitamin of thiamine and a transketolase (TK) inhibitor. Oxythiamine inhibits cancer cell proliferation and induces cell cycle arrest. Oxythiamine has potential anticancer activity, inhibits non-oxidative synthesis of ribose, and induces apoptosis.
Targets&IC50	MIAPaCa2 cells:14.95 μM
In vitro	Oxythiamine for MIA PaCa-2 cells 5-320 μM for 2 days IC50 is 14.95 μM MTT, MIA PaCa-2 cells concentrations of Oxythiamine (0 μM, 5 μM, 50 μM and 500 μM) for 48 hours two-dimensional gel electrophoresis (2DE). Oxythiamine altered protein expression in a dose dependent manner.[1] The MIC and MFC values of Oxythiamine were in the range 0.08 × 103 to 10 × 103 mg/L. [2] A549 cells were co-cultured with Oxythiamine (from 0.1 to 100 μM) for 6–12 h CCK-8 assay Oxythiamine contributes to a significant reduction of A549 cell viability of 11.7%, 23.6%, and 28.2% at 12, 24, and 48 h. [3] Oxythiamine arrests the cell growth in G1 phase of cell cycle. [3] Oxythiamine inhibits the growth of A549 cells by initially slowing-down cell cycle and by leading to apoptosis. [3]
In vivo	s.c. injection C57BL/6 mice with LLC cells and supplemented the mice with a low- or a high-dose of Oxythiamine (250 or 500 mg/kg BW) daily for 5 wk. During the 5-wk period, Oxythiamine supplementation decreased plasma MMP-2 activity in a dose-dependent manner, and this effect was significant after 4 wk of tumor cell implantation. [4]
Synonyms	Hydroxythiamin

Chemical Properties

Molecular Weight	266.34
Formula	C₁₂H₁₆N₃O₂S
Cas No.	136-16-3
Smiles	O=C1NC(=NC=C1C[N+]2=CSC(=C2C)CCO)C
Relative Density.	1.2416 g/cm3 (Estimated)

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (150.18 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.51 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7546 mL	18.7730 mL	37.5460 mL	187.7300 mL
5 mM	0.7509 mL	3.7546 mL	7.5092 mL	37.5460 mL
10 mM	0.3755 mL	1.8773 mL	3.7546 mL	18.7730 mL
20 mM	0.1877 mL	0.9386 mL	1.8773 mL	9.3865 mL
50 mM	0.0751 mL	0.3755 mL	0.7509 mL	3.7546 mL
100 mM	0.0375 mL	0.1877 mL	0.3755 mL	1.8773 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc