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NVP-2

Name: NVP-2
Brand: TargetMol
SKU: T16363
Price: 50 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T16363Cas No. 1263373-43-8

NVP-2 is an effective and selective ATP-competitive cyclin-dependent kinase 9 (CDK9) probe. NVP-2 induces cell apoptosis. NVP-2 inhibits CDK9/CycT activity (IC50: 0.514 nM). NVP-2 shows inhibitory effcts on CDK1/CycB, CDK2/CycA and CDK16/CycY kinases (IC50: 0.584 μM, 0.706 μM, and 0.605 μM, respectively).

NVP-2

🥰Excellent

Purity: 99.13%

Catalog No. T16363Cas No. 1263373-43-8

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$50	In Stock	In Stock
2 mg	$72	In Stock	In Stock
5 mg	$122	In Stock	In Stock
10 mg	$213	In Stock	In Stock
25 mg	$463	In Stock	In Stock
50 mg	$683	In Stock	In Stock
100 mg	$973	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$137	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.13%

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	NVP-2 is an effective and selective ATP-competitive cyclin-dependent kinase 9 (CDK9) probe. NVP-2 induces cell apoptosis. NVP-2 inhibits CDK9/CycT activity (IC50: 0.514 nM). NVP-2 shows inhibitory effcts on CDK1/CycB, CDK2/CycA and CDK16/CycY kinases (IC50: 0.584 μM, 0.706 μM, and 0.605 μM, respectively).
Targets&IC50	CDK9:0.5 nM (IC50)
In vitro	NVP-2 exhibits anti-proliferative activity against leukemia cells, specifically targeting KOPT-K1, Jurkat, P12-ICHIKAWA, DU.528, MOLT 16, HSB-2, PF-382, SKW-3, SUP-T11, DND-41, and HPB-ALL cells with IC50 values of 0.1688 μM, 0.1233 μM, 0.5736 μM, 0.1575 μM, 0.1620 μM, 0.1585 μM, 0.1808 μM, 0.2589 μM, 0.0918 μM, and 0.3023 μM, respectively. NVP-2 (0-10 nM; 72 hours) demonstrates CRBN-dependent anti-proliferative and pro-apoptotic effects in MOLT4 cells (IC50: 9 nM). At 250 nM for 24 hours, NVP-2 induces apoptosis in MOLT4 cells, increasing caspase-3 and γH2A.X expression, although this effect is significantly reduced upon compound washout. Additionally, NVP-2 (250 nM-1 μM; 6 hours) engages CDK9 in both wildtype and CRBN - / - MOLT4 cells without affecting CDK2 and CDK7.

Chemical Properties

Molecular Weight	513.07
Formula	C₂₇H₃₇ClN₆O₂
Cas No.	1263373-43-8
Smiles	COC[C@@H](C)N[C@H]1CC[C@@H](CC1)Nc1cc(c(Cl)cn1)-c1cccc(NCC2(CCOCC2)C#N)n1
Relative Density.	1.23 g/cm3 (Predicted)
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (194.91 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (7.8 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9491 mL	9.7453 mL	19.4905 mL	97.4526 mL
5 mM	0.3898 mL	1.9491 mL	3.8981 mL	19.4905 mL
10 mM	0.1949 mL	0.9745 mL	1.9491 mL	9.7453 mL
20 mM	0.0975 mL	0.4873 mL	0.9745 mL	4.8726 mL
50 mM	0.0390 mL	0.1949 mL	0.3898 mL	1.9491 mL
100 mM	0.0195 mL	0.0975 mL	0.1949 mL	0.9745 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc