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ZMC1

Name: ZMC1
Brand: TargetMol
SKU: T2464
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2464Cas No. 71555-25-4

NSC-319726 (ZMC1), a mutant p53R175 reactivator, suppresses growth of fibroblasts that expresses the p53R175 mutation (IC50 = 8 nM).

ZMC1

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Purity: 99.76%

Catalog No. T2464Cas No. 71555-25-4

NSC-319726 (ZMC1), a mutant p53R175 reactivator, suppresses growth of fibroblasts that expresses the p53R175 mutation (IC50 = 8 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$32	In Stock	In Stock
5 mg	$52	In Stock	In Stock
10 mg	$85	In Stock	In Stock
25 mg	$159	In Stock	In Stock
50 mg	$288	In Stock	In Stock
100 mg	$490	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$57	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.76%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	NSC-319726 (ZMC1), a mutant p53R175 reactivator, suppresses growth of fibroblasts that expresses the p53R175 mutation (IC50 = 8 nM).
Targets&IC50	p53 (R175):8 nM
In vitro	In cells with mutant p53, NSC319726 (IC50=8 nM) causes a WT-like conformational change in the mutant protein that repairs sequence-specific p53 transcription.
In vivo	In cells with mutant p53, NSC319726 (IC50=8 nM) causes a WT-like conformational change in the mutant protein that repairs sequence-specific p53 transcription.
Kinase Assay	MTS: 5,000 cells of TOV112D cells (5,000 cells/well, in 100 ml culture) are cultured in 96-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compounds. The growth is measured by MTS reagent and Victor Plate reader instrument after incubation for 3 days.
Cell Research	Viability assays are done. The cells (5×104 cells/well, in 1 ml culture) are cultured in a 12-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compound. The growth is measured by Guava ViaCount reagent and Guava PCA instrument after incubation for 3 days.(Only for Reference)

Chemical Properties

Molecular Weight	234.32
Formula	C₁₁H₁₄N₄S
Cas No.	71555-25-4
Smiles	C\C(=N/NC(=S)N1CCC1)c1ccccn1
Relative Density.	1.26g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 2.3 mg/mL (9.82 mM), Heating is recommended.

DMSO: 23.4 mg/mL (99.86 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.2677 mL	21.3383 mL	42.6767 mL	213.3834 mL
5 mM	0.8535 mL	4.2677 mL	8.5353 mL	42.6767 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.4268 mL	2.1338 mL	4.2677 mL	21.3383 mL
20 mM	0.2134 mL	1.0669 mL	2.1338 mL	10.6692 mL
50 mM	0.0854 mL	0.4268 mL	0.8535 mL	4.2677 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc