Shopping Cart

Your shopping cart is currently empty

NS-2028

Name: NS-2028
Brand: TargetMol
SKU: T16344
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T16344Cas No. 204326-43-2

NS-2028 is a highly selective soluble Guanylyl Cyclase (sGC) inhibitor (IC50: 30 nM and 200 nM for basal and NO-stimulated enzyme activity) commonly used in nitric oxide signaling pathway research. It inhibits sGC activity in mouse cerebellum homogenates and neuronal NO synthase (IC50: 17 nM and 20 nM), inhibits 3-morpholino-sydnonimine (SIN-1)-elicited cyclic GMP formation in human cultured umbilical vein endothelial cells (IC50: 30 nM), and completely inhibits NO-dependent relaxant responses in non-vascular smooth muscle (1 μM). NS-2028 also decreases vascular endothelial growth factor-induced angiogenesis and permeability.

NS-2028

🥰Excellent

Purity: 99.44%

Catalog No. T16344Cas No. 204326-43-2

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$33	In Stock	In Stock
5 mg	$80	In Stock	In Stock
10 mg	$146	In Stock	In Stock
25 mg	$263	In Stock	In Stock
50 mg	$397	In Stock	In Stock
100 mg	$572	In Stock	In Stock
200 mg	$777	-	In Stock
1 mL x 10 mM (in DMSO)	$89	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.44%

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	NS-2028 is a highly selective soluble Guanylyl Cyclase (sGC) inhibitor (IC50: 30 nM and 200 nM for basal and NO-stimulated enzyme activity) commonly used in nitric oxide signaling pathway research. It inhibits sGC activity in mouse cerebellum homogenates and neuronal NO synthase (IC50: 17 nM and 20 nM), inhibits 3-morpholino-sydnonimine (SIN-1)-elicited cyclic GMP formation in human cultured umbilical vein endothelial cells (IC50: 30 nM), and completely inhibits NO-dependent relaxant responses in non-vascular smooth muscle (1 μM). NS-2028 also decreases vascular endothelial growth factor-induced angiogenesis and permeability.
Targets&IC50	sGC:30 nM
In vitro	NS-2028 (10 μM; 30 mins) decreases VEGF-induced EC migration by inhibiting p38 MAPK activation. NS-2028 (10 μM; 24 hours) inhibits 25% cell number in comparation with those grown in the presence of a vehicle[2].
In vivo	In the avascular rabbit cornea, NS-2028 (p.o; 1 g/L; 8 days) displays an obvious reduction of new vessel formation in response to VEGF pellet implants[2].

Chemical Properties

Molecular Weight	269.05
Formula	C₉H₅BrN₂O₃
Cas No.	204326-43-2
Smiles	Brc1ccc2OCc3noc(=O)n3-c2c1
Relative Density.	2.06 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (929.20 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (18.58 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7168 mL	18.5839 mL	37.1678 mL	185.8391 mL
5 mM	0.7434 mL	3.7168 mL	7.4336 mL	37.1678 mL
10 mM	0.3717 mL	1.8584 mL	3.7168 mL	18.5839 mL
20 mM	0.1858 mL	0.9292 mL	1.8584 mL	9.2920 mL
50 mM	0.0743 mL	0.3717 mL	0.7434 mL	3.7168 mL
100 mM	0.0372 mL	0.1858 mL	0.3717 mL	1.8584 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc