Neophytadiene is a diterpene compound extracted from Turbinaria ornate, with anti-inflammatory, antidepressant, anxiolytic-like, anticonvulsant, antioxidant, and cardioprotective effects. It inhibits LPS-induced inflammation in RAW 264.7 macrophages and Sprague-Dawley rats and can be used in neurological disease research.

Neophytadiene has an IC50 value of 50 μM for RAW 264.7 cells.
Neophytadiene (25, 50, and 100 μM) pretreated LPS-induced RAW 264.7 cells to inhibit NO production and down-regulate the production of inducable nitric oxide synthase (iNOS).
Neophytadiene (25, 50, and 100 μM) significantly reduced the production of TNF-α and NF-κB proteins in LPS-induced cells. [1]

Neophytadiene (12, 25, and 50 mg/kg, orally administered once daily for 7 days) showed that Neophytadiene improved CKMB levels in LPS treated rats in a dose-dependent manner. Restores SOD and catalase activity and reduces levels of pro-inflammatory cytokines such as IL1β, IL6 and IL10. [1]