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MALT1 inhibitor MI-2

Name: MALT1 inhibitor MI-2
Brand: TargetMol
SKU: T2350
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2350Cas No. 1047953-91-2

Alias MI 2 (MALT1 inhibitor), MI 2, MALT1 inhibitor

MI-2 (MALT1 inhibitor) is an irreversible MALT1 inhibitor.

MALT1 inhibitor MI-2

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🥰Excellent

Purity: 99.87%

Catalog No. T2350Alias MI 2 (MALT1 inhibitor), MI 2, MALT1 inhibitorCas No. 1047953-91-2

MI-2 (MALT1 inhibitor) is an irreversible MALT1 inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$31	In Stock	In Stock
5 mg	$98	In Stock	In Stock
10 mg	$129	In Stock	In Stock
25 mg	$218	In Stock	In Stock
50 mg	$328	In Stock	In Stock
100 mg	$538	In Stock	In Stock
200 mg	$766	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$98	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.87%

Appearance:Solid

Color:White

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	MI-2 (MALT1 inhibitor) is an irreversible MALT1 inhibitor.
Targets&IC50	MALT1:5.84 μM
In vitro	Daily intraperitoneal injection of 25 mg/kg MI-2 effectively inhibits the growth of TMD8 and HBL-1 ABC-DLBCL xenografts, while it has no effect on the growth of OCI-Ly1 tumors.
In vivo	MI-2 demonstrates excellent cellular permeability and inhibits the function of MALT1 in the ABC-DLBCL cell lines. It selectively inhibits the growth of MALT1-dependent cell lines, with GI50 values of 0.2, 0.5, 0.4, and 0.4 μM in HBL-1, TMD8, OCI-Ly3, and OCI-Ly10 cells, respectively. Conversely, ABC-DLBCL cell lines not dependent on MALT1, such as U2932 and HLY-1, along with two types of GCB-DLBCL cell lines, exhibit resistance to MI-2.
Kinase Assay	High-Throughput Screening for MALT1 Proteolytic Activity Inhibitors: Ac-LRSR-AMC is used as substrate and reactions are measured with excitation/emission wavelengths of 360/465 nm. Two time points are measured for each reaction to eliminate false positives due to compound autofluorescence. The final percent inhibition is calculated with the formula {[fluorescencetest compound (T2-T1)-fluorescenceneg ctrl (T2-T1)]/[fluorescencepos ctrl (T2-T1)-fluorescenceneg ctrl (T2-T1)]} × 100, where Z-VRPR-FMK (300 nM) is used as positive control and buffer only as negative control. The positive hits are validated in concentration-response experiments within a dose range of 0.122–62.5 μM to determine IC50 of the compounds. Activity is validated using recombinant full-length wild-type MALT1.
Cell Research	Cell proliferation is determined by ATP quantification using a luminescent method and trypan blue dye exclusion. Standard curves for each cell line are calculated by plotting the cell number (determined using trypan blue) against their luminescence values, and cell number is calculated accordingly. Cell viability in drug-treated cells is normalized to their respective controls (fractional viability), and results are given as 1-fractional viability. CompuSyn software is used to determine GI25 and GI50 values.(Only for Reference)
Synonyms	MI 2 (MALT1 inhibitor), MI 2, MALT1 inhibitor

Chemical Properties

Molecular Weight	455.72
Formula	C₁₉H₁₇Cl₃N₄O₃
Cas No.	1047953-91-2
Smiles	COCCOc1nc(-c2ccc(Cl)c(Cl)c2)n(n1)-c1ccc(NC(=O)CCl)cc1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (274.29 mM), Sonication is recommended.

Ethanol: 9.1 mg/mL (19.97 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.39 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1943 mL	10.9716 mL	21.9433 mL	109.7165 mL
5 mM	0.4389 mL	2.1943 mL	4.3887 mL	21.9433 mL
10 mM	0.2194 mL	1.0972 mL	2.1943 mL	10.9716 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1097 mL	0.5486 mL	1.0972 mL	5.4858 mL
50 mM	0.0439 mL	0.2194 mL	0.4389 mL	2.1943 mL
100 mM	0.0219 mL	0.1097 mL	0.2194 mL	1.0972 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc