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GRL-0496

Name: GRL-0496
Brand: TargetMol
SKU: T9672
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T9672Cas No. 1087243-14-8

GRL-0496 is a potent inhibitor with an enzyme inhibitory activity against SARS-CoV 3CLpro with an IC50 of 30 nM and antiviral potency with an EC50 value of 6.9 μM.

GRL-0496

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Purity: 99.9%

Catalog No. T9672Cas No. 1087243-14-8

GRL-0496 is a potent inhibitor with an enzyme inhibitory activity against SARS-CoV 3CLpro with an IC50 of 30 nM and antiviral potency with an EC50 value of 6.9 μM.

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$38	In Stock	In Stock
5 mg	$60	In Stock	In Stock
10 mg	$98	In Stock	In Stock
25 mg	$208	In Stock	In Stock
50 mg	$359	In Stock	In Stock
100 mg	$533	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$67	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.9%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

GRL-0496 AI Summary

GRL-0496 exhibits potent antiviral activity against various strains of SARS-CoV and SARS-CoV-2. In african green monkey Vero E6 cells, it shows an IC50 of 30.0 nM against SARS-CoV Urbani and an EC50 of 2800.0 nM against SARS-CoV-2 WK-521, as measured by qPCR analysis after 3 days. In Vero E6 cells infected with SARS-CoV-2 JPN/TY/WK-251, it exhibits an EC50 of 2800.0 nM, with full antiviral activity observed at concentrations ranging from 1 to 100 µM. Additionally, GRL-0496 effectively inhibits viral replication in human Calu-3 cells and Vero 76 cells, with EC50 values of 28.5 nM and 9.59 nM, respectively. It also targets the SARS-CoV-2 3CL protease with varying IC50 values: 250.0 nM, 313.0 nM when expressed in HEK cells, and 320.0 nM measured by a FRET assay after 5 hours. Moreover, it shows inhibition of SARS 3Clpro with an EC50 of 6900.0 nM. GRL-0496 exhibits high cytotoxicity with a CC50 greater than 100,000.0 nM..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	GRL-0496 is a potent inhibitor with an enzyme inhibitory activity against SARS-CoV 3CLpro with an IC50 of 30 nM and antiviral potency with an EC50 value of 6.9 μM.
Targets&IC50	SARS-CoV:6.9 μM(EC50), 3CLpro (SARS CoV):30 nM

Chemical Properties

Molecular Weight	272.69
Formula	C₁₄H₉ClN₂O₂
Cas No.	1087243-14-8
Smiles	Clc1cncc(OC(=O)c2cccc3[nH]ccc23)c1
Relative Density.	1.431 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (366.72 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (14.67 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6672 mL	18.3358 mL	36.6717 mL	183.3584 mL
5 mM	0.7334 mL	3.6672 mL	7.3343 mL	36.6717 mL
10 mM	0.3667 mL	1.8336 mL	3.6672 mL	18.3358 mL
20 mM	0.1834 mL	0.9168 mL	1.8336 mL	9.1679 mL
50 mM	0.0733 mL	0.3667 mL	0.7334 mL	3.6672 mL
100 mM	0.0367 mL	0.1834 mL	0.3667 mL	1.8336 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc