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DNA2 inhibitor C5

Name: DNA2 inhibitor C5
Brand: TargetMol
SKU: T15146
Price: 55 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15146Cas No. 35973-25-2

DNA2 inhibitor C5 is a promising lead compound to develop sensitizers for cancer chemotherapeutics that cause replication stress and it also is a competitive and specific inhibitor of DNA2 nuclease activity (IC50: 20 μM). DNA2 inhibitor C5 inhibits nuclease, DNA dependent ATPase, helicase, and DNA binding activities of DNA2.

DNA2 inhibitor C5

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Purity: 99.05%

Catalog No. T15146Cas No. 35973-25-2

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$55	In Stock	In Stock
5 mg	$122	In Stock	In Stock
10 mg	$198	In Stock	In Stock
25 mg	$372	In Stock	In Stock
50 mg	$553	In Stock	In Stock
100 mg	$768	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$121	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.05%

Appearance:Solid

Color:Yellow

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COA LCMS HNMR

Product Introduction

DNA2 inhibitor C5 AI Summary

DNA2 inhibitor C5 exhibits a broad range of bioactivities against various biological targets. As an inhibitor, it demonstrates significant activity against HIV-1 integrase, inhibiting 3'-processing (46.9%) and strand transfer (54.9%) at 100 ug/mL. It also inhibits Dengue virus type 2 NS5 RNA methyltransferase SAM site with an IC50 of 14300.0 nM, although its antiviral activity against Dengue virus type 2 in human A549 cells is not significant (EC50 > 100000.0 nM). The compound shows cytotoxicity against human A549 cells with a CC50 > 100000.0 nM and exhibits varying levels of cytotoxicity against other human cell lines, with IC50 values from 7200.0 nM to 95500.0 nM. In addition to antiviral properties, DNA2 inhibitor C5 inhibits JMJD2A-Tudor Domain, HP1-beta Chromodomain, Polymerase Iota, Polymerase Eta, Glutaminase (GLS), WRN Helicase, Pin1, and human tyrosyl-DNA phosphodiesterase 1 (TDP1). It induces DNA re-replication in MCF 10a normal breast cells and shows significant inhibition of nuclease activity and binding affinity to flag-tagged recombinant DNA2, with IC50 and Ki ranging from 20000.0 nM to >250000.0 nM. Furthermore, the compound inhibits helicase activity in DNA2 mutants (3.0%-64.0%), exhibits cytotoxicity in MCF7 cells with an IC50 of 1900.0 nM, and disrupts DNA replication fork restart in A549 cells treated with HU and CPT, exhibiting 60.0% and 50.0% activity respectively. Moreover, DNA2 inhibitor C5 induces cell cycle arrest in human HCT116 cells, causing accumulation in the G1 phase at concentrations of 10 uM (66.9%) and 50 uM (54.1%). It also shows inhibitory effects on Scp-1 phosphatase and caspase 6 based on IC50 and AC50 values. However, it does not significantly inhibit T-cell receptor (TCR)-CD3 interactions..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	DNA2 inhibitor C5 is a promising lead compound to develop sensitizers for cancer chemotherapeutics that cause replication stress and it also is a competitive and specific inhibitor of DNA2 nuclease activity (IC50: 20 μM). DNA2 inhibitor C5 inhibits nuclease, DNA dependent ATPase, helicase, and DNA binding activities of DNA2.
Targets&IC50	DNA2 nuclease:20 μM

Chemical Properties

Molecular Weight	234.16
Formula	C₁₀H₆N₂O₅
Cas No.	35973-25-2
Smiles	OC(=O)c1cnc2c(cccc2c1O)[N+]([O-])=O
Relative Density.	1.624g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 31.25 mg/mL (133.46 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.2706 mL	21.3529 mL	42.7058 mL	213.5292 mL
5 mM	0.8541 mL	4.2706 mL	8.5412 mL	42.7058 mL
10 mM	0.4271 mL	2.1353 mL	4.2706 mL	21.3529 mL
20 mM	0.2135 mL	1.0676 mL	2.1353 mL	10.6765 mL
50 mM	0.0854 mL	0.4271 mL	0.8541 mL	4.2706 mL
100 mM	0.0427 mL	0.2135 mL	0.4271 mL	2.1353 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc