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Dihydrostreptomycin sulfate

Name: Dihydrostreptomycin sulfate
Brand: TargetMol
SKU: T2211
Price: 45 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2211Cas No. 5490-27-7

Alias Dihydrostreptomycin sesquisulfate

Dihydrostreptomycin sulfate (Dihydrostreptomycin sesquisulfate) inhibits protein synthesis by binding to the 30S ribosomal subunit. Dihydrostreptomycin Sulfate is a semi-synthetic aminoglycoside antibiotic with bactericidal properties. This antibiotic is active against most gram-positive and gram-negative organisms and is used in the treatment of tuberculosis and tularemia.

Dihydrostreptomycin sulfate

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Purity: 99.9%

Catalog No. T2211Alias Dihydrostreptomycin sesquisulfateCas No. 5490-27-7

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 g	$45	-	In Stock

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Purity:99.9%

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Dihydrostreptomycin sulfate (Dihydrostreptomycin sesquisulfate) inhibits protein synthesis by binding to the 30S ribosomal subunit. Dihydrostreptomycin Sulfate is a semi-synthetic aminoglycoside antibiotic with bactericidal properties. This antibiotic is active against most gram-positive and gram-negative organisms and is used in the treatment of tuberculosis and tularemia.
In vitro	The primary mechanism of action of the antibiotic dihydrostreptomycin is binding to and modifying the function of the bacterial ribosome, thus leading to decreased and aberrant translation of proteins. It enters bacterial cells by binding to a specific site on MscL(mechanosensitive channel of large conductance) and modifying its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell[1].
In vivo	Dihydrostreptomycin sulfate would cause cochlea damage in mouse model and cell proliferation in the spiral ligament may occur after the dihydrostreptomycin sulfate -induced damage. This process is probably related to the recovery of cochlear function[2].
Kinase Assay	Briefly, the peptide substrate N-acetyl-Asp-Glu-Val-Asp-ρ-nitroanilide (Ac-DEVD-ρNA) is added to the cell lysates in assay buffer (50 mM HEPES, pH 7.4, 100 mM NaCl, 0.1% CHAPS, 10 mM dithiothreitol, 1 mM EDTA, 10% glycerol) and incubated at 37°C. The cleavage of the substrate is monitored at 405 nm.
Synonyms	Dihydrostreptomycin sesquisulfate

Chemical Properties

Molecular Weight	730.71
Formula	C₂₁H₄₁N₇O₁₂·1·5H₂SO₄
Cas No.	5490-27-7
Smiles	OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]2NC(N)=N)O[C@@H](C)[C@]1(O)CO.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]2NC(N)=N)O[C@@H](C)[C@]1(O)CO
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 10 mM, Sonication is recommended.

DMSO: Insoluble

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	1.3685 mL	6.8427 mL	13.6853 mL	68.4266 mL
5 mM	0.2737 mL	1.3685 mL	2.7371 mL	13.6853 mL
10 mM	0.1369 mL	0.6843 mL	1.3685 mL	6.8427 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc