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D-I03

Name: D-I03
Brand: TargetMol
SKU: T10936
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: DI03) Copy Product Info

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Synonyms: DI03

Catalog No. T10936 Copy Product Info

Purity: 99.65%

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D-I03 is a selective RAD52 inhibitor with a Kd of 25.8 µM. D-I03 inhibits RAD52-dependent single-strand annealing (SSA) and D-loop formation, with IC₅₀ values of 5 µM and 8 µM, respectively. D-I03 exerts significant growth inhibition on BRCA1/2-deficient cancer cells but has no effect on RAD51. D-I03 can be used in combination therapy studies for tumors with DNA damage repair defects.

Cas No. 688342-78-1

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Pack Size	Price	USA Stock	Global Stock
1 mg	$31	In Stock	In Stock
5 mg	$70	In Stock	In Stock
10 mg	$128	In Stock	In Stock
25 mg	$263	In Stock	In Stock
50 mg	$392	In Stock	In Stock
100 mg	$575	In Stock	In Stock
200 mg	$818	In Stock	-
1 mL x 10 mM (in DMSO)	$77	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.65%

Appearance:Solid

Color:Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	D-I03 is a selective RAD52 inhibitor with a Kd of 25.8 µM. D-I03 inhibits RAD52-dependent single-strand annealing (SSA) and D-loop formation, with IC₅₀ values of 5 µM and 8 µM, respectively. D-I03 exerts significant growth inhibition on BRCA1/2-deficient cancer cells but has no effect on RAD51. D-I03 can be used in combination therapy studies for tumors with DNA damage repair defects.
Targets & IC50	RAD52:25.8 µM (Ki), single-chain annealing (SSA):5 µM, D-loop:8 µM
In vitro	Methods: In BRCA1-mutant UWB1.298 and MDA-MB-436 cells, medium containing different concentrations of D-I03 (0 μM, 2.5 μM, 5 μM, and 10 μM) was added on days 1 and 3, respectively. On day 4, cells were harvested and viable cells were counted using the trypan blue exclusion Methods. An equal number of viable cells were then subcultured into fresh 6-well plates and cultured in drug-free normal medium for approximately 2 weeks to allow single cells to proliferate and form colonies. Results: D-I03 significantly inhibited colony formation in a dose-dependent manner within the concentration range of 2.5–10 μM.[1]
In vivo	Methods: Using a BRCA2-deficient, PARP inhibitor-resistant ovarian cancer C57BL/6 mouse model, mice received olaparib (75 mg/kg, oral infusion), D-I03 (25 mg/kg, intraperitoneal injection), olaparib plus D-I03, or vehicle treatment for 20 days. Results: Combination therapy with D-I03 and PARP inhibitors reduced tumor burden and prolonged survival.[2]
Synonyms	DI03

Chemical Properties

Molecular Weight	428.64
Formula	C₂₃H₃₆N₆S
Cas No.	688342-78-1
Smiles	CCN(CC)CCNC(=S)Nc1ccc2nc(cc(C)c2c1)N1CCN(CC)CC1
Relative Density.	1.147 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (209.97 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (7.7 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3330 mL	11.6648 mL	23.3296 mL	116.6480 mL
5 mM	0.4666 mL	2.3330 mL	4.6659 mL	23.3296 mL
10 mM	0.2333 mL	1.1665 mL	2.3330 mL	11.6648 mL
20 mM	0.1166 mL	0.5832 mL	1.1665 mL	5.8324 mL
50 mM	0.0467 mL	0.2333 mL	0.4666 mL	2.3330 mL
100 mM	0.0233 mL	0.1166 mL	0.2333 mL	1.1665 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc