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CE3F4

Name: CE3F4
Brand: TargetMol
SKU: T12616L
Price: 47 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12616LCas No. 143703-25-7

CE3F4 is a selective antagonist of exchange protein directly activated by cAMP (Epac1; IC50s of 10.7 μM and 66 μM for Epac1 and Epac2(B), respectively).

CE3F4

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Purity: 98.06%

Catalog No. T12616LCas No. 143703-25-7

CE3F4 is a selective antagonist of exchange protein directly activated by cAMP (Epac1; IC50s of 10.7 μM and 66 μM for Epac1 and Epac2(B), respectively).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$47	In Stock	In Stock
10 mg	$80	In Stock	In Stock
25 mg	$156	In Stock	In Stock
50 mg	$288	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$52	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:98.06%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	CE3F4 is a selective antagonist of exchange protein directly activated by cAMP (Epac1; IC50s of 10.7 μM and 66 μM for Epac1 and Epac2(B), respectively).
Targets&IC50	EPAC2B:66 μM, EPAC1:10.7 μM
In vitro	CE3F4 acts as a selective antagonist of the Epac1, demonstrating inhibitory concentrations (IC50) of 10.7 μM for Epac1 and 66 μM for Epac2(B), illustrating greater potency against Epac1 as compared to its (S)-stereoisomer ((S)-CE3F4, IC50, 56 μM), yet showing lesser activity than its (R)-stereoisomer ((R)-CE3F4, IC50, 5.8 μM). At a concentration of 20 μM, CE3F4 effectively inhibits Epac-induced Rap1 activation in HEK293 cells and significantly reduces the late-phase ERK activation prompted by glucose in INS-1 cells. Moreover, at 40 μM, it specifically targets Epac1's guanine nucleotide exchange activity without disturbing Rap1's function or the Epac1-Rap1 interaction. CE3F4 at a 50 μM concentration exhibits stronger inhibitory effects on the guanine nucleotide exchange factor (GEF) activity of Epac1 compared to both forms of Epac2. It also inhibits the Epac1 exchange activity induced by compound 007, with an IC50 of 23 ± 3 μM, while not affecting PKA activity.

Chemical Properties

Molecular Weight	351.01
Formula	C₁₁H₁₀Br₂FNO
Cas No.	143703-25-7
Smiles	CC1CCc2c(Br)c(F)c(Br)cc2N1C=O
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (142.45 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.7 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8489 mL	14.2446 mL	28.4892 mL	142.4461 mL
5 mM	0.5698 mL	2.8489 mL	5.6978 mL	28.4892 mL
10 mM	0.2849 mL	1.4245 mL	2.8489 mL	14.2446 mL
20 mM	0.1424 mL	0.7122 mL	1.4245 mL	7.1223 mL
50 mM	0.0570 mL	0.2849 mL	0.5698 mL	2.8489 mL
100 mM	0.0285 mL	0.1424 mL	0.2849 mL	1.4245 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc