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BI8622

Name: BI8622
Brand: TargetMol
SKU: T14568
Rating: 4.5 (1 reviews)

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Catalog No. T14568Cas No. 1875036-74-0

Alias BI 8622

BI8622 is a ubiquitin ligase HUWE1-specific antagonist that restores the level of RP2 mutants in retinal cell lines and inhibits the activation of NLRP3, NLRC4, and AIM2 inflammatory vesicles in cells.

BI8622

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Purity: 98.28%

Catalog No. T14568Alias BI 8622Cas No. 1875036-74-0

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$116	6-8 weeks	6-8 weeks
5 mg	$297	6-8 weeks	6-8 weeks
25 mg	$987	6-8 weeks	6-8 weeks
50 mg	$1,360	6-8 weeks	6-8 weeks
100 mg	$1,860	6-8 weeks	6-8 weeks
1 mL x 10 mM (in DMSO)	$365	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.28%

Appearance:Solid

Color:White to Yellow

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Product Introduction

Bioactivity

Description	BI8622 is a ubiquitin ligase HUWE1-specific antagonist that restores the level of RP2 mutants in retinal cell lines and inhibits the activation of NLRP3, NLRC4, and AIM2 inflammatory vesicles in cells.
Targets&IC50	HUWE1:3.1 μM, Ls174T cells:8.4 μM, MCL1:6.8 μM
In vitro	BI8622 is a specific inhibitor of HUWE1 ubiquitin ligase with an IC50 of 3.1 μM.It inhibited ubiquitination of MCL1 in HeLa cells by ectopic expression of HUWE1, with an IC50 value of 6.8 μM.In addition, BI8622 inhibited clonogenic formation of Ls174T cells with an estimated IC50 of 8.4 μM. BI8622 (10 μM, 1-4 days treatment) delayed the progression of Ls174T cells at all stages of the cell cycle, with the most pronounced inhibitory effect in the G1 phase.BI8622 (0-50 μM, 16 hours treatment) prevented the degradation of MCL1 in HeLa cells under UV irradiation by inhibiting HUWE1. It also inhibited MYC-dependent transcriptional activation in colorectal cancer cells. [1]
Synonyms	BI 8622

Chemical Properties

Molecular Weight	426.51
Formula	C₂₅H₂₆N₆O
Cas No.	1875036-74-0
Smiles	N#CC1(C=2C=CC=CC2)CCN(C=3N=CN=C(C(=O)NC4=CC=C(C=C4)CN)C3C)CC1
Relative Density.	1.29 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (234.46 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (9.38 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3446 mL	11.7231 mL	23.4461 mL	117.2305 mL
5 mM	0.4689 mL	2.3446 mL	4.6892 mL	23.4461 mL
10 mM	0.2345 mL	1.1723 mL	2.3446 mL	11.7231 mL
20 mM	0.1172 mL	0.5862 mL	1.1723 mL	5.8615 mL
50 mM	0.0469 mL	0.2345 mL	0.4689 mL	2.3446 mL
100 mM	0.0234 mL	0.1172 mL	0.2345 mL	1.1723 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc