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BI8622

Name: BI8622
Brand: TargetMol
SKU: T14568
Rating: 4.5 (1 reviews)

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Catalog No. T14568Cas No. 1875036-74-0

Alias BI 8622

BI8622 is a ubiquitin ligase HUWE1-specific antagonist that restores the level of RP2 mutants in retinal cell lines and inhibits the activation of NLRP3, NLRC4, and AIM2 inflammatory vesicles in cells.

BI8622

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Purity: 98.28%

Catalog No. T14568Alias BI 8622Cas No. 1875036-74-0

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$116	6-8 weeks	6-8 weeks
5 mg	$297	6-8 weeks	6-8 weeks
25 mg	$987	6-8 weeks	6-8 weeks
50 mg	$1,360	6-8 weeks	6-8 weeks
100 mg	$1,860	6-8 weeks	6-8 weeks
1 mL x 10 mM (in DMSO)	$365	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.28%

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Product Introduction

Bioactivity

Description	BI8622 is a ubiquitin ligase HUWE1-specific antagonist that restores the level of RP2 mutants in retinal cell lines and inhibits the activation of NLRP3, NLRC4, and AIM2 inflammatory vesicles in cells.
Targets&IC50	Ls174T cells:8.4 μM, MCL1:6.8 μM, HUWE1:3.1 μM
In vitro	BI8622 is a specific inhibitor of HUWE1 ubiquitin ligase with an IC50 of 3.1 μM.It inhibited ubiquitination of MCL1 in HeLa cells by ectopic expression of HUWE1, with an IC50 value of 6.8 μM.In addition, BI8622 inhibited clonogenic formation of Ls174T cells with an estimated IC50 of 8.4 μM. BI8622 (10 μM, 1-4 days treatment) delayed the progression of Ls174T cells at all stages of the cell cycle, with the most pronounced inhibitory effect in the G1 phase.BI8622 (0-50 μM, 16 hours treatment) prevented the degradation of MCL1 in HeLa cells under UV irradiation by inhibiting HUWE1. It also inhibited MYC-dependent transcriptional activation in colorectal cancer cells. [1]
Synonyms	BI 8622

Chemical Properties

Molecular Weight	426.51
Formula	C₂₅H₂₆N₆O
Cas No.	1875036-74-0
Smiles	N#CC1(C=2C=CC=CC2)CCN(C=3N=CN=C(C(=O)NC4=CC=C(C=C4)CN)C3C)CC1
Relative Density.	1.29 g/cm3 (Predicted)
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (234.46 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (9.38 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3446 mL	11.7231 mL	23.4461 mL	117.2305 mL
5 mM	0.4689 mL	2.3446 mL	4.6892 mL	23.4461 mL
10 mM	0.2345 mL	1.1723 mL	2.3446 mL	11.7231 mL
20 mM	0.1172 mL	0.5862 mL	1.1723 mL	5.8615 mL
50 mM	0.0469 mL	0.2345 mL	0.4689 mL	2.3446 mL
100 mM	0.0234 mL	0.1172 mL	0.2345 mL	1.1723 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc