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ATG7-IN-1

Name: ATG7-IN-1
Brand: TargetMol
SKU: T39705
Price: 329 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: ATG7-IN-1) Copy Product Info

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Synonyms: ATG7-IN-1

Catalog No. T39705 Copy Product Info

Purity: 99.85%

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ATG7-IN-1 is a highly potent, selective small-molecule ATG7 inhibitor with an IC₅₀ of 62 nM. ATG7-IN-1 blocks the formation of the ATG12-ATG5 and LC3-PE conjugate system at both the cellular and in vivo levels, thereby inhibiting autophagosome formation. ATG7-IN-1 can be used in research on autophagy mechanisms.

Cas No. 2226229-87-2

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Pack Size	Price	USA Stock	Global Stock
1 mg	$329	In Stock	In Stock
5 mg	$798	In Stock	In Stock
10 mg	$1,090	In Stock	In Stock
25 mg	$1,650	In Stock	In Stock
50 mg	$2,230	In Stock	In Stock
100 mg	$2,930	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.85%

Appearance:Solid

Color:White

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COA FNMR HNMR LCMS

Product Introduction

ATG7-IN-1 AI Summary

ATG7-IN-1 exhibits significant bioactivity profiles across various cellular assays. It shows potent inhibition of recombinant His-tagged human ATG7 with an IC50 of 62.0 nM. Additionally, it induces autophagy in human H4 cells (IC50 = 659.0 nM) and in human SKOV3 cells with different markers: an EC50 of 9400.0 nM based on NRB1 accumulation and an EC50 of 3000.0 nM based on p62 accumulation. The compound also demonstrates antiproliferative activity against human H1650 cells with an EC50 greater than 50000.0 nM. Lastly, it inhibits UAE, NAE, and SAE enzymes, each with an IC50 greater than 1000.0 nM..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	ATG7-IN-1 is a highly potent, selective small-molecule ATG7 inhibitor with an IC₅₀ of 62 nM. ATG7-IN-1 blocks the formation of the ATG12-ATG5 and LC3-PE conjugate system at both the cellular and in vivo levels, thereby inhibiting autophagosome formation. ATG7-IN-1 can be used in research on autophagy mechanisms.
Targets & IC50	SKOV3 cells:3 μM (EC50), NCI-H1650 cells:> 50 μM (EC50), ATG7:62 nM
In vitro	Methods: H4 glioma cells were treated with ATG7-IN-1 (0.01, 0.03, 0.1, 0.3, 1, 3 μmol/L) for 6 hours. LC3B spot formation was quantified by immunofluorescence to assess the inhibition of autophagic flux. Results: ATG7-IN-1 significantly reduced the number of LC3B foci and inhibited LC3 phosphorylation downstream of ATG7, with an IC₅₀ as low as the micromolar range. [1]
In vivo	Methods: HCT116 colorectal cancer xenograft mice were administered 150 mg/kg of ATG7-IN-1 via subcutaneous injection. Tumor tissue expression of LC3B and NBR1 was assessed 4 hours after dosing. Results: ATG7-IN-1 effectively inhibited autophagy in tumor tissue in vivo, reduced LC3B phosphorylation, and induced NBR1 accumulation, demonstrating good in vivo efficacy. [1]
Synonyms	ATG7-IN-1

Chemical Properties

Molecular Weight	470.5
Formula	C₁₇H₁₉FN₆O₅S₂
Cas No.	2226229-87-2
Smiles	S(C=1C=2C(N(N1)[C@@H]3O[C@@H](COS(N)(=O)=O)[C@@H](O)C3)=NC=NC2N)C4=C(F)C=C(C)C=C4

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 255 mg/mL (541.98 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (10.63 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1254 mL	10.6270 mL	21.2540 mL	106.2699 mL
5 mM	0.4251 mL	2.1254 mL	4.2508 mL	21.2540 mL
10 mM	0.2125 mL	1.0627 mL	2.1254 mL	10.6270 mL
20 mM	0.1063 mL	0.5313 mL	1.0627 mL	5.3135 mL
50 mM	0.0425 mL	0.2125 mL	0.4251 mL	2.1254 mL
100 mM	0.0213 mL	0.1063 mL	0.2125 mL	1.0627 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc