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Anti-ATG7 Polyclonal Antibody

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Catalog No. TMAB-00164

Anti-ATG7 Polyclonal Antibody is a Rabbit antibody targeting ATG7. Anti-ATG7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ATG7 Polyclonal Antibody

Anti-ATG7 Polyclonal Antibody

😃Good
Catalog No. TMAB-00164
Anti-ATG7 Polyclonal Antibody is a Rabbit antibody targeting ATG7. Anti-ATG7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ATG7 Polyclonal Antibody is a Rabbit antibody targeting ATG7. Anti-ATG7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse)
Verified Activity
1. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-APG7 (TMAB-00164) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kDa
Observed band size: 85 kDa
2. Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-APG7 Polyclonal Antibody, Unconjugated (TMAB-00164) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm (Probable).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ATG7
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Energy Metabolism,Cancer,Mitophagy fission and fusion,Autophagy and mitophagy,APG gene products,Energy Metabolism,Ub-like Proteins,Associated Proteins,APG gene products,Autophagy,Signal Transduction,APG gene products
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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