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Anti-ATG7 Polyclonal Antibody is a Rabbit antibody targeting ATG7. Anti-ATG7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-ATG7 Polyclonal Antibody is a Rabbit antibody targeting ATG7. Anti-ATG7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse) |
Verified Activity | 1. Sample: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-APG7 (TMAB-00164) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 85 kDa 2. Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-APG7 Polyclonal Antibody, Unconjugated (TMAB-00164) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm (Probable). |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]. |
Immunogen | KLH conjugated synthetic peptide: human ATG7 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Energy Metabolism,Cancer,Mitophagy fission and fusion,Autophagy and mitophagy,APG gene products,Energy Metabolism,Ub-like Proteins,Associated Proteins,APG gene products,Autophagy,Signal Transduction,APG gene products |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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