Anti-ATG7 Polyclonal Antibody is a Rabbit antibody targeting ATG7. Anti-ATG7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse)

1. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-APG7 (TMAB-00164) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kDa
Observed band size: 85 kDa
2. Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-APG7 Polyclonal Antibody, Unconjugated (TMAB-00164) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ATG7

Human

ATG7

Ubiquitin-like modifier-activating enzyme ATG7

Energy Metabolism,Cancer,Mitophagy fission and fusion,Autophagy and mitophagy,APG gene products,Energy Metabolism,Ub-like Proteins,Associated Proteins,APG gene products,Autophagy,Signal Transduction,APG gene products

Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3.