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W146

Catalog No. T13327 CAS 909725-61-7

Synonyms: W 146, W-146

W146 is a selective sphingosine-1-phosphate receptor 1 (S1PR1) antagonist (EC50: 398 nM).

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W146, CAS 909725-61-7

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1 mg	35 days	$ 540.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	W146 is a selective sphingosine-1-phosphate receptor 1 (S1PR1) antagonist (EC50: 398 nM).
Targets&IC50	S1PR1:(EC50)398 nM
In vitro	W146 is an antagonist of S1PR1 (Ki of ~70-80 nM)[1]. The S1P attenuated TUNEL labeling index of EPCs is significantly increased by pretreatment with W146 compared with the S1P treated group. Furthermore, pretreatment with W146 significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146[2].
In vivo	In mice, Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count . when mice are pretreated with W146, it is found that a significant increase in KSL-HSPC mobilization , compared to that in mice pretreated with dextran followed by AMD3100 administration. Moreover, pre-treatment of W146 shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone[3].

Synonyms	W 146, W-146
Molecular Weight	342.37
Formula	C16H27N2O4P
CAS No.	909725-61-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. M Germana Sanna, et al. Enhancement of capillary leakage and restoration of lymphocyte egress by a chiral S1P1 antagonist in vivo. Nat Chem Biol. 2006 Aug;2(8):434-41. Epub 2006 Jul 9. 2. Hang Wang, et al. Sphingosine-1-phosphate promotes the proliferation and attenuates apoptosis of Endothelial progenitor cells via S1PR1/S1PR3/PI3K/Akt pathway. Cell Biol Int. 2018 May 23. 3. Jingjing Liu, et al. 3-amino-4-(3-hexylphenylamino)-4-oxobutyl phosphonic acid (W146), a Selective Antagonist of Sphingosine-1-phospahte Receptor Subtype 1, Enhances AMD3100-stimulated Mobilization of Hematopoietic Stem Progenitor Cells in Animals. J Biochem Pharmacol Res. 2013 Dec; 1(4): 197–203.

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Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

W146 909725-61-7 Others W 146 W-146 inhibitor inhibit

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