Home Tools

Tideglusib

Catalog No. T3067 CAS 865854-05-3

Synonyms: NP-12, NP031112

Tideglusib (NP031112), a non-ATP competitive inhibitor of glycogen synthase kinase 3 (GSK-3), is with anti-inflammatory and neuroprotective activities.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Tideglusib, CAS 865854-05-3

Pack Size	Availability	Price/USD
10 mg	In stock	$ 39.00
25 mg	In stock	$ 70.00
50 mg	In stock	$ 115.00
100 mg	In stock	$ 197.00
200 mg	In stock	$ 297.00
500 mg	In stock	$ 516.00
1 mL * 10 mM (in DMSO)	In stock	$ 43.00

Bulk Inquiry

Select Batch

Purity: 99.84%

Purity: 99%

Purity: 98.89%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Tideglusib (NP031112), a non-ATP competitive inhibitor of glycogen synthase kinase 3 (GSK-3), is with anti-inflammatory and neuroprotective activities.
Targets&IC50	GSK-3β:60 nM
Kinase Assay	[35S]Tideglusib (207 Bq/nmol) at 55 μM is incubated with 5 μM GSK-3β for 1 h at 25°C in 315 μL of 50 mM Tris-HCl, pH 7.5, containing 150 mM NaCl and 0.1 mM EGTA. The incubation is extended for another 30 min after having added 35 μL of the same buffer with or without 100 mM DTE. Samples are then processed in three different ways. First, an aliquot of 125 μL of each sample is mixed with 375 μL of 8 M GdnHCl in Water and heated at 80°C for 5 min. A second aliquot of 125 μL is diluted up to 500 μL with Water and left at room temperature for 5 min. In both cases, the free drug is removed afterwards by gel filtration through Sephadex G-25, and the amount of bound drug is determined by liquid scintillation counting on a 1450-MicroBeta TriLux counter. Finally, a third 40 μL aliquot of each original sample is mixed with 10 μL of denaturing electrophoresis sample buffer without reducing agents, and 35 μL of this mixture is loaded onto a 10% polyacrylamide gel and subjected to SDS-PAGE (again in the absence of reducing agents except for the DTE already included in the corresponding sample), followed by fluorography of the dried gel[1].

Synonyms	NP-12, NP031112
Molecular Weight	334.39
Formula	C19H14N2O2S
CAS No.	865854-05-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: >15 mg/mL(44.9 mM)

References and Literature

1. Domínguez JM, et al. J Biol Chem, 2012, 287(2), 893-90 2. Luna-Medina R, et al. J Neurosci, 2007, 27(21), 5766-5776. 3. Serenó L, et al. Neurobiol Dis, 2009, 35(3), 359-367.

Citations

1. Dou X, Sun Q, Xu G, et al. Discovery of 2-(furan-2-ylmethylene) hydrazine-1-carbothioamide derivatives as novel inhibitors of SARS-CoV-2 main protease. European Journal of Medicinal Chemistry. 2022: 114508

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Highly Selective Inhibitor Library Inhibitor Library Anti-Cancer Clinical Compound Library Kinase Inhibitor Library FDA-Approved Kinase Inhibitor Library Anti-Cancer Drug Library Anti-Neurodegenerative Disease Compound Library Anti-Cancer Active Compound Library Anti-Cancer Approved Drug Library

Related Products

Related compounds with same targets

EHT 1610 IM-12 AR-A014418 Indirubin 5-Iodo-indirubin-3'-monoxime Laduviglusib trihydrochloride ALSTERPAULLONE 4,10-Aromadendranediol

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Tideglusib 865854-05-3 PI3K/Akt/mTOR signaling Stem Cells GSK-3 Glycogen synthase kinase-3 NP 031112 NP-12 NP 12 inhibit Glycogen synthase kinase 3 NP12 Inhibitor NP-031112 NP031112 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.