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TOFA

Catalog No. T3988 CAS 54857-86-2

Synonyms: MDL14514, RMI14514

TOFA (MDL14514) is an allosteric inhibitor of acetyl-CoA carboxylase-α (ACCA).

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TOFA, CAS 54857-86-2

Pack Size	Availability	Price/USD
2 mg	In stock	$ 32.00
5 mg	In stock	$ 48.00
10 mg	In stock	$ 63.00
25 mg	In stock	$ 117.00
50 mg	In stock	$ 197.00
100 mg	In stock	$ 372.00
200 mg	In stock	$ 549.00
500 mg	In stock	$ 869.00
1 mL * 10 mM (in DMSO)	In stock	$ 48.00

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Purity: 99.77%

Purity: 99.71%

Biological Description

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Storage & Solubility Information

Description	TOFA (MDL14514) is an allosteric inhibitor of acetyl-CoA carboxylase-α (ACCA).
In vitro	TOFA (5-tetradecyloxy-2-furoic acid) effectively inhibits the proliferation of examined cancer cells by inducing apoptosis, arresting them in the G0/G1 cell cycle phase, and exhibiting time and dose-dependent cytotoxic effects. It specifically targets Acetyl-CoA-carboxylase-α (ACCA), a crucial enzyme in fatty acid synthesis regulation, leading to decreased fatty acid synthesis, caspase activation, and cell death, particularly in prostate cancer (PCa) cell lines. Additionally, TOFA demonstrates significant cytotoxicity toward lung cancer cells NCI-H460 and colon carcinoma cells HCT-8 and HCT-15, showcasing IC50 values around 5.0, 5.0, and 4.5 μg/mL respectively. At concentrations ranging from 1.0 to 20.0 μg/mL, it blocks fatty acid synthesis and prompts cell death in a dose-dependent manner. Furthermore, TOFA has proven cytotoxic effects on COC1 and COC1/DDP cells, with IC50 values approximately at 26.1 and 11.6 μg/mL, respectively.
In vivo	The tumor growth rate is signifi?cantly inhibited by TOFA compared with the DMSO treated control mice (1649±356.3 vs. 5128±390.4 mm3. No toxicity is observed in the heart, liver, spleen, lung, kidney and intestinal tissues. TOFA inhibits COC1/DDP cell growth in ovarian tumor mouse xenografts. By inhibiting ACC, TOFA may be a promising small molecule agent for ovarian cancer therapy.
Cell Research	NCI-H460, human lung cancer cells, and HCT-8 and HCT-15 cells (5,000/well) are seeded in 96-well plates overnight and then exposed to TOFA at indicated concentrations (0, 1, 5, 10, 20, 50 μg/mL) for 72 hours. Viable cells are detected using MTT assay[1].

Synonyms	MDL14514, RMI14514
Molecular Weight	324.45
Formula	C19H32O4
CAS No.	54857-86-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 6.88 mg/mL (21.19 mM)

References and Literature

1. Wang C, et al. Acetyl-CoA carboxylase-alpha inhibitor TOFA induces human cancer cell apoptosis. Biochem Biophys Res Commun. 2009 Jul 31;385(3):302-6. 2. Li S, et al. TOFA suppresses ovarian cancer cell growth in vitro and in vivo. Mol Med Rep. 2013 Aug;8(2):373-8. 3. Guseva NV, et al. TOFA (5-tetradecyl-oxy-2-furoic acid) reduces fatty acid synthesis, inhibits expression of AR, neuropilin-1 and Mcl-1 and kills prostate cancer cells independent of p53 status. Cancer Biol Ther. 2011 Jul 1;12(1):80-5.

Citations

1. Chen S, Ni J, Luo L, et al.Toosendanin induces hepatotoxicity via disrupting LXRα/Lipin1/SREBP1 mediated lipid metabolism.Food and Chemical Toxicology.2024: 114631.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compounds Library Max Anti-Hypertension Compound Library ReFRAME Related Library Anti-Cardiovascular Disease Compound Library Bioactive Lipid Compound Library Anti-Metabolism Disease Compound Library NO PAINS Compound Library Bioactive Compound Library Anti-Obesity Compound Library

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Keywords

TOFA 54857-86-2 Metabolism Acetyl-CoA Carboxylase MDL14514 inhibit RMI-14514 ACC, Acetyl Coenzyme A Carboxylase RMI 14514 Inhibitor RMI14514 MDL-14514 MDL 14514 inhibitor

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