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Anti-ACACA Antibody (3I598)
😃Good
Catalog No. TMAB-00029
Anti-ACACA Antibody (3I598) is a Rabbit antibody targeting ACACA. Anti-ACACA Antibody (3I598) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ACACA Antibody (3I598)
Anti-ACACA Antibody (3I598)
😃Good
Catalog No. TMAB-00029
Anti-ACACA Antibody (3I598) is a Rabbit antibody targeting ACACA. Anti-ACACA Antibody (3I598) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $153 | 7-10 days | |
| 50 μL | $271 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Clone | 3I598 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by microwaving in EDTA buffer (pH8.0) for 5 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Acetyl CoA Carboxylase 1 (ACC1) Monoclonal Antibody, Unconjugated (TMAB-00029) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwaving in EDTA buffer (pH8.0) for 5 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Acetyl CoA Carboxylase 1 (ACC1) Monoclonal Antibody, Unconjugated (TMAB-00029) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Mouse Brain Lysates Lane 2: Human K562 cell Lysates Primary: Anti-Acetyl CoA Carboxylase 1 (ACC1) (TMAB-00029) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 265 kDa Observed band size: 265 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Acetyl CoA Carboxylase 1(ACC1) |
| Antigen Species | Human |
| Gene ID | 32 |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,Metabolism of lipids and lipoproteins,Synthesis,Integration of energy,Lipid metabolism,Mitochondrial markers,Fatty acid oxidation,Lipid metabolism,Mitochondrial |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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