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Pladienolide B

Catalog No. T16551 CAS 445493-23-2

Pladienolide B is a potent spliceosome inhibitor, a macrolide isolated from Streptomyces obtusususus Mer-12, which targets the SF3B1 subunit of the spliceosome.Pladienolide B exerts its antitumor effects by inhibiting pre-mRNA splicing and inducing necrosis.Pladienolide B possesses antitumor activity and can be used to study leukemia and lymphoid tumors. Pladienolide B has antitumor activity and can be used to study leukemia and lymphoid tumors.

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Pladienolide B, CAS 445493-23-2

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100 μg	In stock	$ 347.00

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Description	Pladienolide B is a potent spliceosome inhibitor, a macrolide isolated from Streptomyces obtusususus Mer-12, which targets the SF3B1 subunit of the spliceosome.Pladienolide B exerts its antitumor effects by inhibiting pre-mRNA splicing and inducing necrosis.Pladienolide B possesses antitumor activity and can be used to study leukemia and lymphoid tumors. Pladienolide B has antitumor activity and can be used to study leukemia and lymphoid tumors.
In vitro	Pladienolide B (0.1-2 nM; 24-48 h; HeLa cells) decreases SF3b1 expression in human cervical carcinoma cells. Pladienolide B causes (0.1-2 nM; 24 h; HeLa cells) cell cycle arrest and apoptosis. Pladienolide B (0.1-2 nM; 24-72 h; HeLa cells) suppresses human cervical carcinoma cells viability. Pladienolide B Significantly decreased cell viability, and the decrease was concentration- and time-dependent.[3]
In vivo	Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days; BALB/c nu/nu mice; PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts) showed strong growth inhibitory or regressive activities against these xenografts. Having intense antitumor activities.[4]

Molecular Weight	536.7
Formula	C30H48O8
CAS No.	445493-23-2

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Effenberger KA, et al. Coherence between cellular responses and in vitro splicing inhibition for the anti-tumor drug pladienolide B and its analogs. J Biol Chem. 2014 Jan 24;289(4):1938-47. 2. Aouida M, et al. CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B. Biochim Open. 2016 Feb 24;3:72-75. 3. Zhang Q, et al. Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1273-1280. 4. Mizui Y, et al. Pladienolides, new substances from culture of Streptomyces platensis Mer-11107. III. In vitro and in vivo antitumor activities. J Antibiot (Tokyo). 2004 Mar;57(3):188-96.

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Keywords

Pladienolide B 445493-23-2 Apoptosis inhibitor inhibit

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