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PFM01

Catalog No. T8880 CAS 1558598-41-6

PFM01 is an inhibitor of MRE11 endonuclease. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR).

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PFM01, CAS 1558598-41-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 39.00
10 mg	In stock	$ 64.00
25 mg	In stock	$ 123.00
50 mg	In stock	$ 228.00
100 mg	In stock	$ 372.00
200 mg	In stock	$ 517.00
1 mL * 10 mM (in DMSO)	In stock	$ 43.00

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Purity: 99.76%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

PFM01 is an inhibitor of MRE11 endonuclease. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR).

In vitro

PFM01 (100 μM) treatment allows normal DSB repair in HR-defective ATMi-treated or HSC62 (BRCA2-defective) cells[1].
RAD51 foci did not form following exo- or endo-inhibitor treatment (mirin or PFM01) in 1BR3 (WT) and HSC62 (BRCA2-defective) cells[1].
PFM01 (100 μM) enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells[1].
PFM01 substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells[1].

Molecular Weight	293.4
Formula	C14H15NO2S2
CAS No.	1558598-41-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 4 mg/mL (13.63 mM)

References and Literature

1. Shibata A, et, al. DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Mol Cell. 2014 Jan 9; 53(1): 7-18. 2. Völkening L, et, al. RAD50 regulates mitotic progression independent of DNA repair functions. FASEB J. 2020 Feb; 34(2): 2812-2820.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compounds Library Max DNA Damage & Repair Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PFM01 1558598-41-6 Others Inhibitor derivative MRE11 NHEJ Mirin DSB endonuclease PFM 01 N-alkylated PFM-01 inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.