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Deoxyribonuclease II
🥰Excellent
Catalog No. TRP-00291Cas No. 9025-64-3
Alias DNaseII, DNase 2, Deoxyribonuclease 2
Deoxyribonuclease II from porcine spleen (DNase II) is a nucleic acid endonuclease primarily located within lysosomes. It cleaves the phosphodiester bond in DNA between the 5'-phosphate and 3'-hydroxyl groups, degrading exogenous DNA and generating nucleoside-3'-phosphate.
Deoxyribonuclease II
Deoxyribonuclease II
🥰Excellent
Catalog No. TRP-00291Alias DNaseII, DNase 2, Deoxyribonuclease 2Cas No. 9025-64-3
Deoxyribonuclease II from porcine spleen (DNase II) is a nucleic acid endonuclease primarily located within lysosomes. It cleaves the phosphodiester bond in DNA between the 5'-phosphate and 3'-hydroxyl groups, degrading exogenous DNA and generating nucleoside-3'-phosphate.
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Color:White
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| Description | Deoxyribonuclease II from porcine spleen (DNase II) is a nucleic acid endonuclease primarily located within lysosomes. It cleaves the phosphodiester bond in DNA between the 5'-phosphate and 3'-hydroxyl groups, degrading exogenous DNA and generating nucleoside-3'-phosphate. |
| In vitro | During the purification process of β-lactamase, Deoxyribonuclease II was used to treat transformed cells [2]. In the study investigating the effect of growth hormone secretagogue (somatoprim) on growth hormone secretion in human adenoma cell cultures (hSA), Deoxyribonuclease II was employed for preparing adenoma tissues [3]. |
| Synonyms | DNaseII, DNase 2, Deoxyribonuclease 2 |
| Cas No. | 9025-64-3 |
| Relative Density. | no data available |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility Information | H2O: ≥ 4 mg/mL, Sonication is recommended. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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