Deoxyribonuclease II from porcine spleen (DNase II) is a nucleic acid endonuclease primarily located within lysosomes. It cleaves the phosphodiester bond in DNA between the 5'-phosphate and 3'-hydroxyl groups, degrading exogenous DNA and generating nucleoside-3'-phosphate.

During the purification process of β-lactamase, Deoxyribonuclease II was used to treat transformed cells [2]. In the study investigating the effect of growth hormone secretagogue (somatoprim) on growth hormone secretion in human adenoma cell cultures (hSA), Deoxyribonuclease II was employed for preparing adenoma tissues [3].