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PF-431396

PF-431396
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Purity:99.82%
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PF-431396

Catalog No. T2314Cas No. 717906-29-1
PF-431396 is a dual PYK2/FAK inhibitor (IC50: 11/2 nM).
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
5 mg$50In Stock
10 mg$74In Stock
25 mg$148In Stock
50 mg$261In Stock
100 mg$447In Stock
200 mg$659In Stock
1 mL x 10 mM (in DMSO)$59In Stock
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Product Introduction

Bioactivity
Description
PF-431396 is a dual PYK2/FAK inhibitor (IC50: 11/2 nM).
In vivo
PF-431396 consistently inhibits protein tyrosine kinase phosphorylation under both calcium deficiency and the presence of Ca/W-7. It also blocks tyrosine phosphorylation of Pyk2 and FAK induced by anti-Ig- and aggregated LFA-1 in A20 cells, thereby inhibiting B cell proliferation.
Kinase Assay
In Vitro Kinase Assays and Selectivity Screening : To measure Mps1 activity, 25 ng of recombinant, full-length enzyme is incubated in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 0.01% Triton X-100 and 5 μM Myelin basic protein (MBP)) containing vehicle (DMSO alone) or inhibitors. Forty μM ATP (2xKm) is added with 1 μCi [γ-33P]ATP and the reaction is incubated at room temperature for 45 minutes. Reactions are terminated with 3% phosphoric acid and transferred to P81 filter plates. Samples are washed in 1% phosphoric acid and 33P radioactivity is measured on a TopCount scintillation reader. In-house kinase assays are all carried out at 2xKm ATP concentrations. MPI-0479605 (500 nM) is also screened against a larger kinase panel.
Chemical Properties
Molecular Weight506.5
FormulaC22H21F3N6O3S
Cas No.717906-29-1
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 25.3 mg/mL (50 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.9743 mL9.8717 mL19.7433 mL98.7167 mL
5 mM0.3949 mL1.9743 mL3.9487 mL19.7433 mL
10 mM0.1974 mL0.9872 mL1.9743 mL9.8717 mL
20 mM0.0987 mL0.4936 mL0.9872 mL4.9358 mL

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