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PF-431396

Catalog No. T2314 CAS 717906-29-1

Synonyms: PF 431396

PF-431396 is a dual PYK2/FAK inhibitor (IC50: 11/2 nM).

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PF-431396, CAS 717906-29-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PF-431396 is a dual PYK2/FAK inhibitor (IC50: 11/2 nM).
Targets&IC50	FAK:2 nM, PYK2:11 nM
Kinase Assay	In Vitro Kinase Assays and Selectivity Screening : To measure Mps1 activity, 25 ng of recombinant, full-length enzyme is incubated in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 0.01% Triton X-100 and 5 μM Myelin basic protein (MBP)) containing vehicle (DMSO alone) or inhibitors. Forty μM ATP (2xKm) is added with 1 μCi [γ-33P]ATP and the reaction is incubated at room temperature for 45 minutes. Reactions are terminated with 3% phosphoric acid and transferred to P81 filter plates. Samples are washed in 1% phosphoric acid and 33P radioactivity is measured on a TopCount scintillation reader. In-house kinase assays are all carried out at 2xKm ATP concentrations. MPI-0479605 (500 nM) is also screened against a larger kinase panel.

Synonyms	PF 431396
Molecular Weight	506.5
Formula	C22H21F3N6O3S
CAS No.	717906-29-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 25.3 mg/mL (50 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Han S, et al. J Biol Chem. 2009, 284(19), 13193-13201. 2. Tse KW, et al. J Biol Chem. 2009, 284(34), 22865-22877. 3. González-Fernández L, et al. Biol Reprod. 2013, 88(6), 13

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Inhibitor Library Fluorochemical Library Kinase Inhibitor Library Cytoskeletal Signaling Pathway Compound Library Angiogenesis related Compound Library Bioactive Compound Library Tyrosine Kinase Inhibitor Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PF-431396 717906-29-1 蛋白酪氨酸激酶细胞骨架血管生成 FAK PYK2 inhibit Inhibitor Proline-rich tyrosine kinase 2 PF431396 Pyk2 PF 431396 PTK2 Focal adhesion kinase PTK2 protein tyrosine kinase 2 inhibitor

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