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Org 27569

Org 27569
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Org 27569

Catalog No. T2635Cas No. 868273-06-7
Org 27569, an allosteric modulator of cannabinoid CB1 receptor, can induce a CB1 receptor state that is characterized by decreased inverse agonist affinity and enhanced agonist affinity.
With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.
Pack SizePriceAvailabilityQuantity
2 mg$31In Stock
5 mg$48In Stock
10 mg$81In Stock
25 mg$159In Stock
50 mg$297In Stock
100 mg$443In Stock
1 mL x 10 mM (in DMSO)$48In Stock
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Product Introduction

Org 27569, an allosteric modulator of cannabinoid CB1 receptor, can induce a CB1 receptor state that is characterized by decreased inverse agonist affinity and enhanced agonist affinity.
In vivo
ORG 27569 (3.2 and 5.6 mg/kg, intraperitoneally) significantly reduces both cue-induced and priming-induced reinstatement of cocaine and methamphetamine in rats[4]. At a dosage of 30 mg/kg, intraperitoneally, Org27569 induces appetite suppression independent of CB1 receptors and does not alter the discriminative stimulus effects of anandamide (AEA). Furthermore, an intracerebroventricular administration of Org27569 (100 μg) does not influence the pharmacological effects of CP55,940 administered systemically when compared to a vehicle[5].
Kinase Assay
Equilibrium Binding Assays.: Binding assays are performed with the CB1 receptor agonist [3H]CP 55,940 (0.7 nM) and the CB1 receptor antagonist [3H]SR 141716A (1.2 nM), 1 mg/ml BSA and 50 mM Tris buffer containing 0.1 mM EDTA and 0.5 mM MgCl2, pH 7.4, in a total assay volume of 500 μl. Binding is initiated by the addition of mouse brain membranes (30 μg). Assays are carried out at 37°C for 60 min before termination by addition of ice-cold wash buffer (50 mM Tris buffer and 1 mg/ml BSA) and vacuum filtration using a 24-well sampling manifold and Whatman GF/B glass-fiber filters that have been soaked in wash buffer at 4°C for 24 h. Each reaction tube is washed five times with a 4-ml aliquot of buffer. The filters are oven-dried for 60 min and then placed in 5 ml of scintillation fluid, and radioactivity is quantitated by liquid scintillation spectrometry. Specific binding is defined as the difference between the binding that occurred in the presence and absence of 1 μM concentrations of the corresponding unlabeled ligand and is 70 to 80% of the total binding.
Cell Research
Cells expressing CB1 receptors are exposed to ORG27569 (10 μM) for 5 to 15 min. For toxin treatment to abrogate Gi coupling effects, PTX is added to the medium at 5 ng/ml. Following an 18-h incubation in the presence of toxin, cells are washed twice with PBS and treated with compounds. Cells are washed with ice-cold PBS, and cell lysates are obtained by harvesting the cells with ice-cold lysis buffer (150 mM NaCl, 1.0% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% SDS, and 50 mM Tris, pH 7.5 containing 4-(2-aminoethyl)benzenesulfonyl fluoride, pepstatin A, E-64, bestatin, leupeptin, and aprotinin as protease inhibitors).(Only for Reference)
Chemical Properties
Molecular Weight409.95
Cas No.868273-06-7
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 41 mg/mL (100 mM)
Solution Preparation Table
1 mM2.4393 mL12.1966 mL24.3932 mL121.9661 mL
5 mM0.4879 mL2.4393 mL4.8786 mL24.3932 mL
10 mM0.2439 mL1.2197 mL2.4393 mL12.1966 mL
20 mM0.1220 mL0.6098 mL1.2197 mL6.0983 mL
50 mM0.0488 mL0.2439 mL0.4879 mL2.4393 mL
100 mM0.0244 mL0.1220 mL0.2439 mL1.2197 mL


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