Anti-CNR1 Polyclonal Antibody is a Rabbit antibody targeting CNR1. Anti-CNR1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CNR1) Polyclonal Antibody, Unconjugated (TMAB-00452) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-CNR1 (TMAB-00452) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
3. Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat liver);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min;Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CNR1) Polyclonal Antibody, Unconjugated (TMAB-00452) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat hippocampus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CNR1) Polyclonal Antibody, Unconjugated (TMAB-00452) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CNR-1

Human

CNR1

Cannabinoid receptor 1

Lipoprotein metabolism,Cholesterol Metabolism,Cholesterol Metabolism,Lipoprotein metabolism,Heart disease,Cannabinoid Receptor

Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.