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Nucleozin

Catalog No. T7330 CAS 341001-38-5

Nucleozin targets influenza A nucleoprotein (NP), a multifunctional, RNA-binding protein necessary for virus replication.

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Nucleozin, CAS 341001-38-5

Pack Size	Availability	Price/USD
2 mg	In stock	$ 36.00
5 mg	In stock	$ 53.00
10 mg	In stock	$ 85.00
25 mg	In stock	$ 178.00
50 mg	In stock	$ 289.00
100 mg	In stock	$ 428.00
1 mL * 10 mM (in DMSO)	In stock	$ 62.00

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Purity: 98%

Biological Description

Chemical Properties

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Description	Nucleozin targets influenza A nucleoprotein (NP), a multifunctional, RNA-binding protein necessary for virus replication.
In vitro	Nucleozin blocked the cytoplasmic trafficking of ribonucleoproteins (RNPs) that had undergone nuclear export, promoting the formation of large perinuclear aggregates of RNPs along with cellular Rab11. This effect led to the production of much reduced amounts of often markedly smaller virus particles. The primary target of nucleozin is the viral RNP. IAV replication can be effectively inhibited by blocking cytoplasmic trafficking of the viral genome[1].
In vivo	Nucleozin, that triggers the aggregation of NP and inhibits its nuclear accumulation. Nucleozin impeded influenza A virus replication in vitro with a nanomolar median effective concentration (EC(50)) and protected mice challenged with lethal doses of avian influenza A H5N1. Viral NP is a valid target for the development of small-molecule therapies[2].
Cell Research	For live imaging, cells were grown in chambered glass bottom dishes and maintained at 37°C in L-15 CO2-independent medium (Gibco) during analysis. Cells were transfected with GFP-NP and infected with PR8 12 h later. For nucleozin treatment, images were acquired for 5 min and then nucleozin was added to a final concentration of 2 μM before imaging for around 20 to 30 min . Images were acquired at 0.25 or 0.71 frame/s and processed with ImageJ[1].

Molecular Weight	426.85
Formula	C21H19ClN4O4
CAS No.	341001-38-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 28.66 mg/mL (67.14 mM)

References and Literature

1. Amorim M J , Kao R Y , Digard P . Nucleozin Targets Cytoplasmic Trafficking of Viral Ribonucleoprotein-Rab11 Complexes in Influenza A Virus Infection[J]. Journal of Virology, 2013, 87(8):4694-4703. 2. Kao R Y , Yang D , Lau L S , et al. Identification of influenza A nucleoprotein as an antiviral target[J]. Nature Biotechnology, 2010, 28(6):600-605. 3. Zhao, Ming, et al. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature Communications . 12.1 (2021): 1-14.

Citations

1. Zhao, Ming, et al. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature Communications. 2021 Apr 9;12(1):2114. doi: 10.1038/s41467-021-22297-8.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Infection Compound Library Anti-COVID-19 Compound Library Bioactive Compound Library Bioactive Compounds Library Max Immunology/Inflammation Compound Library Anti-Viral Compound Library

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Keywords

Nucleozin 341001-38-5 Microbiology/Virology Influenza Virus inhibit viral Inhibitor infection nuclear nucleoprotein virus replication accumulation inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.