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Neomycin sulfate

Catalog No. T0950 CAS 1405-10-3

Synonyms: Framycin sulfate

Neomycin sulfate (Framycin sulfate) is a broad-spectrum aminoglycoside antibiotic that blocks the synthesis of bacterial proteins in order to exert antimicrobial activity. Neomycin sulfate is commonly used to screen prokaryotic and eukaryotic cells for Neo resistance genes.

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Neomycin sulfate, CAS 1405-10-3

Pack Size	Availability	Price/USD	Quantity
5 g	In stock	$ 43.00
10 g	In stock	$ 50.00

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Purity: 99.87%

Purity: 99.64%

Purity: 99.62%

Purity: 99.57%

Purity: 99.07%

Purity: 98.79%

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Description	Neomycin sulfate (Framycin sulfate) is a broad-spectrum aminoglycoside antibiotic that blocks the synthesis of bacterial proteins in order to exert antimicrobial activity. Neomycin sulfate is commonly used to screen prokaryotic and eukaryotic cells for Neo resistance genes.
In vitro	METHODS: BHK-21 cells, VERO cells and FEA cells were treated with Neomycin sulfate (1000-20000 μg/mL) for 24 h. Cell viability was measured by MTT. RESULTS: The viability of BHK-21 cells was significantly reduced by 9000-20000 μg/mL Neomycin treatment. 3000 μg/mL Neomycin treatment significantly reduced the viability of FEA cells. 20000 μg/mL Neomycin treatment did not affect the viability of VERO cells. [1] METHODS: Mouse cochlear hair cells HEI-OC-1 were treated with Neomycin sulfate (2 mM) for 24 h. Apoptosis was detected by Flow Cytometry. RESULTS: 20 μM GM1 significantly reduced Neomycin sulfate-induced cell death in HEI-OC-1 cells. [2]
In vivo	METHODS: To assay in vivo activity, Neomycin sulfate (20 mg/mouse) and bacitracin (20 mg/mouse) were administered by gavage to C57BL/10 mice once daily for seven days. RESULTS: The combination of Neomycin and bacitracin decreased intestinal permeability and increased gene expression of ZO-1, JAM-A, and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon. [3] METHODS: To deplete the intestinal microbiota of mice, the antibiotics (ABX) vancomycin (0.5 g/L), ampicillin (1 g/L), Neomycin sulfate (1 g/L), and metronidazole (1 g/L) were administered to mice by drinking water for two weeks. RESULTS: Antibiotics significantly reduced the diversity and composition of the gut microbiota. [4]

Synonyms	Framycin sulfate
Molecular Weight	908.87
Formula	C23H46N6O13·3H2SO4
CAS No.	1405-10-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: Insoluble

H2O: 10 mM

References and Literature

1. Kovacik A, et al. Cytotoxic effect of aminoglycoside antibiotics on the mammalian cell lines. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2021;56(1):1-8. 2. Li Y, et al. The Ganglioside Monosialotetrahexosylganglioside Protects Auditory Hair Cells Against Neomycin-Induced Cytotoxicity Through Mitochondrial Antioxidation: An in vitro Study. Front Cell Neurosci. 2021 Sep 27;15:751867. 3. Nevado R, et al. Neomycin and bacitracin reduce the intestinal permeability in mice and increase the expression of some tight-junction proteins. Rev Esp Enferm Dig. 2015 Nov;107(11):672-6. 4. Li F, et al. The intestinal microbial metabolite nicotinamide n-oxide prevents herpes simplex encephalitis via activating mitophagy in microglia. Gut Microbes. 2022 Jan-Dec;14(1):2096989.

Citations

1. Shan T, Huang Y, Zhao Z, et al. Ketogenic diet restrains herpes simplex encephalitis via gut microbes. Microbes and Infection. 2022: 105061. 2. Li F, Wang Y, Song X, et al. The intestinal microbial metabolite nicotinamide n-oxide prevents herpes simplex encephalitis via activating mitophagy in microglia. Gut Microbes. 2022, 14(1): 2096989 3. Zhang Z, Ye J, Liu X, et al.Huangqi Guizhi Wuwu decoction alleviates oxaliplatin-induced peripheral neuropathy via the gut-peripheral nerve axis.Chinese Medicine.2023, 18(1): 1-15.

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Microbial Natural Product Library Antibiotics Library Natural Product Library for HTS Drug-induced Liver Injury (DILI) Compound Library Immunology/Inflammation Compound Library Orally Active Compound Library Anti-infective Natural Product Library Anti-Obesity Compound Library Approved Drug Library

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Keywords

Neomycin sulfate 1405-10-3 Metabolism Microbiology/Virology ribosome Phospholipase Antibacterial Antibiotic Framycin Sulfate Framycin sulfate ribosomal protein Inhibitor proliferation aminoglycoside angiogenin Neomycin synthesis Bacterial Neomycin Sulfate inhibit inhibitor

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