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LY 303511

Catalog No. T2434 CAS 154447-38-8

LY303511, an inactive analogue of LY294002, is a mTOR inhibitor and no inhibition for PI3-K.

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LY 303511, CAS 154447-38-8

Pack Size	Availability	Price/USD
5 mg	4-6 weeks	$ 97.00
10 mg	4-6 weeks	$ 150.00
25 mg	4-6 weeks	$ 330.00
50 mg	4-6 weeks	$ 562.00
100 mg	4-6 weeks	$ 898.00
1 mL * 10 mM (in DMSO)	4-6 weeks	$ 97.00

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	LY303511, an inactive analogue of LY294002, is a mTOR inhibitor and no inhibition for PI3-K.
Targets&IC50	K+currents, in MIN6 insulinoma cells:64.6±9.1 µM (IC50)
Kinase Assay	Mammalian DNA-PK (500 ng/μL) is isolated from HeLa cell nuclear extract after chromatography using Q-Sepharose, S-Sepharose, and Heparin agarose. DNA-PK (250 ng) activity is measured at 30°C, in a final volume of 40 μL, in buffer containing 25 mM HEPES (pH 7.4), 12.5 mM MgCl2, 50 mM KCl, 1 mM DTT, 10% v/v Glycerol, 0.1% w/v NP-40, and 1 mg of the substrate GST-p53N66 (the NH2-terminal 66 amino acid residues of human wild-type p53 fused to glutathione S-transferase) in polypropylene 96-well plates. To the assay mix, varying concentrations of inhibitor (in DMSO at a final concentration of 1% v/v) are added. After 10 min of incubation, ATP is added to give a final concentration of 50 μM, along with a 30-mer double-stranded DNA oligonucleotide (final concentration of 0.5 ng/mL), to initiate the reaction. After 1 h with shaking, 150 μL of PBS are added to the reaction, and 5 μL are then transferred to a 96-well opaque white plate containing 45 μL of PBS per well, where the GSTp53N66 substrate is allowed to bind to the wells for 1 h. To detect the phosphorylation event on the serine 15 residue of p53 elicited by DNA-PK, a p53 phosphoserine-15 antibody is used in a basic ELISA procedure. An antirabbit horseradish peroxidase-conjugated secondary antibody is then used in the ELISA before the addition of chemiluminescence reagent to detect the signal as measured by chemiluminescent counting via a TopCount NXT[1].

Molecular Weight	306.36
Formula	C19H18N2O2
CAS No.	154447-38-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 2 years

Solubility Information

DMSO: 100 mM

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Kristof AS, et al. J Pharmacol Exp Ther. 2005 Sep;314(3):1134-43.

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PI3K/Akt/mTOR-IN-2 N-Ethyl-N′-[4-[4-(8-oxa-3-azabicyclo[3.2.1]oct-3-yl)thieno[3,2-d]pyrimidin-2-yl]phenyl]urea PI3Kα/mTOR-IN-1 Urea, N-[4-[4-(8-oxa-3-azabicyclo[3.2.1]oct-3-yl)thieno[3,2-d]pyrimidin-2-yl]phenyl]-N'-3-pyridinyl- 1-(2-hydroxyethyl)-3-{4-[4-(8-oxa-3-azabicyclo[3.2.1]oct-3-yl)thieno[3,2-d]pyrimidin-2-yl]phenyl}urea 1-(4-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)thieno[3,2-d]pyrimidin-2-yl)phenyl)-3-cyclopropylurea 1-(2-fluoroethyl)-3-{4-[4-(8-oxa-3-azabicyclo[3.2.1]oct-3-yl)thieno[3,2-d]pyrimidin-2-yl]phenyl}urea 1-(4-(4-(8-oxa-3-azabicyclo[3.2.1]octan-3-yl)thieno[3,2-d]pyrimidin-2-yl)phenyl)-3-phenylurea

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

LY 303511 154447-38-8 Apoptosis Membrane transporter/Ion channel PI3K/Akt/mTOR signaling mTOR Potassium Channel TNF KcsA Inhibitor Tumor Necrosis Factor Receptor LY-303511 inhibit TNFR TNF Receptor LY303511 inhibitor

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