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LY 303511

Catalog No. T2434 CAS 154447-38-8

LY303511, an inactive analogue of LY294002, is a mTOR inhibitor and no inhibition for PI3-K.

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LY 303511, CAS 154447-38-8

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	LY303511, an inactive analogue of LY294002, is a mTOR inhibitor and no inhibition for PI3-K.
Targets&IC50	K+currents, in MIN6 insulinoma cells:64.6±9.1 µM (IC50)
Kinase Assay	Mammalian DNA-PK (500 ng/μL) is isolated from HeLa cell nuclear extract after chromatography using Q-Sepharose, S-Sepharose, and Heparin agarose. DNA-PK (250 ng) activity is measured at 30°C, in a final volume of 40 μL, in buffer containing 25 mM HEPES (pH 7.4), 12.5 mM MgCl2, 50 mM KCl, 1 mM DTT, 10% v/v Glycerol, 0.1% w/v NP-40, and 1 mg of the substrate GST-p53N66 (the NH2-terminal 66 amino acid residues of human wild-type p53 fused to glutathione S-transferase) in polypropylene 96-well plates. To the assay mix, varying concentrations of inhibitor (in DMSO at a final concentration of 1% v/v) are added. After 10 min of incubation, ATP is added to give a final concentration of 50 μM, along with a 30-mer double-stranded DNA oligonucleotide (final concentration of 0.5 ng/mL), to initiate the reaction. After 1 h with shaking, 150 μL of PBS are added to the reaction, and 5 μL are then transferred to a 96-well opaque white plate containing 45 μL of PBS per well, where the GSTp53N66 substrate is allowed to bind to the wells for 1 h. To detect the phosphorylation event on the serine 15 residue of p53 elicited by DNA-PK, a p53 phosphoserine-15 antibody is used in a basic ELISA procedure. An antirabbit horseradish peroxidase-conjugated secondary antibody is then used in the ELISA before the addition of chemiluminescence reagent to detect the signal as measured by chemiluminescent counting via a TopCount NXT[1].

Molecular Weight	306.365
Formula	C19H18N2O2
CAS No.	154447-38-8

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 100 mM

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Kristof AS, et al. J Pharmacol Exp Ther. 2005 Sep;314(3):1134-43.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

LY 303511 154447-38-8 PI3K/Akt/mTOR信号通路凋亡离子通道 mTOR Potassium Channel TNF KcsA Inhibitor Tumor Necrosis Factor Receptor LY-303511 inhibit TNFR TNF Receptor LY303511 inhibitor

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