Shopping Cart

Your shopping cart is currently empty

Hygromycin B

Name: Hygromycin B
Brand: TargetMol
SKU: T1022
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

😃Good

Hot

Catalog No. T1022Cas No. 31282-04-9

Alias Hygrovetine

Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic cells by interfering with translocation and causing mistranslation by the 70S ribosome. It is commonly used for the selection of cells transfected with the hph or hyg resistance gene.

Hygromycin B

😃Good

Hot

Purity: 99.78%

Catalog No. T1022Alias HygrovetineCas No. 31282-04-9

Pack Size	Price	USA Warehouse	Global Warehouse
100 mg	$35	In Stock	In Stock
200 mg	$48	In Stock	In Stock
500 mg	$70	-	In Stock
1 mL x 10 mM (in DMSO)	$54	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.78%

Color:White to Orange

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic cells by interfering with translocation and causing mistranslation by the 70S ribosome. It is commonly used for the selection of cells transfected with the hph or hyg resistance gene.
Targets&IC50	E. coli:20 μg/mL (MIC)
In vitro	METHODS: Mouse PDAC cells 14387T were transfected with lentiCRISPRv2 hygro lentivirus, and after 48 h, the transfected cells were cultured in new medium containing Hygromycin B (500 μg/mL) for two weeks, and the successful transfected cells were screened. RESULTS: Hygromycin B screened the lentivirally successfully transfected cells. [1] METHODS: E. coli was cultured in medium containing Hygromycin B (0-8 μg/mL) and cell growth was monitored using a Klett-Summerson colorimeter. RESULTS: Hygromycin B reduced the number of viable cells and increased doubling time in a concentration-dependent manner.Hygromycin B inhibited the number of viable cells by 50% at a concentration of 20 μg/mL, and halved the rate of growth at a concentration of 25 μg/mL. [2]
In vivo	METHODS: To assay antiviral activity in vivo, Hygromycin B (0-5 μmoL/kg) was administered intraperitoneally to MHV-A59-infected BALB/c mice twice daily for three days. RESULTS: Hygromycin B was able to reduce the levels of viral replication and necrotic liver foci in vivo. [3]
Synonyms	Hygrovetine

Chemical Properties

Molecular Weight	527.52
Formula	C₂₀H₃₇N₃O₁₃
Cas No.	31282-04-9
Smiles	CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@@]4(O[C@H]23)O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)[C@@H]1O
Relative Density.	1.67 g/cm3

Storage & Solubility Information

Storage

keep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 240 mg/mL (454.96 mM), Sonication is recommended.

H2O: 125 mg/mL (236.96 mM), Sonication is recommended.

In Vivo Formulation

5% DMSO+95% Saline: 1.58 mg/mL (3 mM), Solution.

Saline: 50 mg/mL (94.78 mM), Solution.

10% DMSO+90% Saline: 3 mg/mL (5.69 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8957 mL	9.4783 mL	18.9566 mL	94.7831 mL
5 mM	0.3791 mL	1.8957 mL	3.7913 mL	18.9566 mL
10 mM	0.1896 mL	0.9478 mL	1.8957 mL	9.4783 mL
20 mM	0.0948 mL	0.4739 mL	0.9478 mL	4.7392 mL
50 mM	0.0379 mL	0.1896 mL	0.3791 mL	1.8957 mL
100 mM	0.0190 mL	0.0948 mL	0.1896 mL	0.9478 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc