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Hygromycin B

Catalog No. T1022 CAS 31282-04-9

Synonyms: Hygrovetine

Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome. Hygromycin B can be used to screen prokaryotic or eukaryotic cells transfected with hph or hyg resistance genes.

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Hygromycin B, CAS 31282-04-9

Pack Size	Availability	Price/USD
200 mg	In stock	$ 48.00
500 mg	In stock	$ 70.00
1 mL * 10 mM (in DMSO)	In stock	$ 54.00

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Purity: 99.9%

Purity: 99.7%

Purity: 99.59%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome. Hygromycin B can be used to screen prokaryotic or eukaryotic cells transfected with hph or hyg resistance genes.
In vitro	METHODS: Mouse PDAC cells 14387T were transfected with lentiCRISPRv2 hygro lentivirus, and after 48 h, the transfected cells were cultured in new medium containing Hygromycin B (500 μg/mL) for two weeks, and the successful transfected cells were screened. RESULTS: Puromycin dihydrochloride screened the lentivirally successfully transfected cells. [1] METHODS: E. coli was cultured in medium containing Hygromycin B (0-8 μg/mL) and cell growth was monitored using a Klett-Summerson colorimeter. RESULTS: Hygromycin B reduced the number of viable cells and increased doubling time in a concentration-dependent manner.Hygromycin B inhibited the number of viable cells by 50% at a concentration of 20 μg/mL, and halved the rate of growth at a concentration of 25 μg/mL. [2]
In vivo	METHODS: To assay antiviral activity in vivo, Hygromycin B (0-5 μmoL/kg) was administered intraperitoneally to MHV-A59-infected BALB/c mice twice daily for three days. RESULTS: Hygromycin B was able to reduce the levels of viral replication and necrotic liver foci in vivo. [3]

Synonyms	Hygrovetine
Molecular Weight	527.52
Formula	C20H37N3O13
CAS No.	31282-04-9

Storage

keep away from moisture

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 30 mg/mL (56.87 mM)

References and Literature

1. Fan M, et al. UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis. Cell Rep. 2024 Feb 16;43(2):113808. 2. McGaha SM, et al. Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli. Antimicrob Agents Chemother. 2007 Feb;51(2):591-6. 3. Macintyre G, et al. Hygromycin B therapy of a murine coronaviral hepatitis. Antimicrob Agents Chemother. 1991 Oct;35(10):2125-7. 4. Santerre RF, et al. Gene, 1984, 30(1-3), 147-156. 5. Hanif M, et al. Curr Genet, 2002, 41(3), 183-188.

Citations

1. Fan M, Huo S, Guo Y, et al.UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis.Cell Reports.2024, 43(2): 113808.

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Antiparasitic Natural Product Library Anti-Parasitic Compound Library Inhibitor Library Microbial Natural Product Library FDA-Approved & Pharmacopeia Drug Library Bioactive Compound Library NO PAINS Compound Library ReFRAME Related Library Natural Product Library for HTS

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Keywords

Hygromycin B 31282-04-9 Microbiology/Virology Antifungal Antibacterial Antibiotic ribosome inhibit Fungal Bacterial Hygrovetine Inhibitor inhibitor

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