Shopping Cart

Your shopping cart is currently empty

GW 4064

Name: GW 4064
Brand: TargetMol
SKU: T2233
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T2233Cas No. 278779-30-9

GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM).

GW 4064

Copy Product Info

😃Good

Purity: 99.94%

Catalog No. T2233Cas No. 278779-30-9

GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$46	In Stock	In Stock
10 mg	$76	In Stock	In Stock
25 mg	$142	In Stock	In Stock
50 mg	$247	In Stock	In Stock
100 mg	$447	In Stock	In Stock
200 mg	$673	In Stock	In Stock
500 mg	$1,060	-	In Stock
1 mL x 10 mM (in DMSO)	$57	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.94%

Color:White

Resource Download

COA HPLC

Product Introduction

Bioactivity

Description	GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM).
Targets&IC50	FXR:65 nM(EC50)
In vitro	In Fisher rats, oral administration of GW 4064 (ED50 = 20 mg/kg) effectively reduces serum triglycerides.
In vivo	In CV-1 cells transfected with mouse FXR expression vectors (EC50=80 nM) and in CV-1 cells transfected with human FXR expression vectors (EC50=90 nM), GW 4064 functions as a selective non-steroidal FXR agonist, effectively exerting agonistic effects.
Cell Research	GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis[2].

Chemical Properties

Molecular Weight	542.84
Formula	C₂₈H₂₂Cl₃NO₄
Cas No.	278779-30-9
Smiles	CC(C)c1onc(c1COc1ccc(C=Cc2cccc(c2)C(O)=O)c(Cl)c1)-c1c(Cl)cccc1Cl
Relative Density.	1.367 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (92.11 mM), Sonication is recommended.

Ethanol: 5.4 mg/mL (9.95 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.68 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8422 mL	9.2108 mL	18.4216 mL	92.1082 mL
5 mM	0.3684 mL	1.8422 mL	3.6843 mL	18.4216 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.1842 mL	0.9211 mL	1.8422 mL	9.2108 mL
20 mM	0.0921 mL	0.4605 mL	0.9211 mL	4.6054 mL
50 mM	0.0368 mL	0.1842 mL	0.3684 mL	1.8422 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc