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GANT 61

Catalog No. T3070 CAS 500579-04-4

Synonyms: GANT61, NSC 136476

GANT 61 is an inhibitor for Gli1 and Gli2.

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GANT 61, CAS 500579-04-4

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	GANT 61 is an inhibitor for Gli1 and Gli2.
Targets&IC50	GLI1:5 μM
Kinase Assay	Dual Luciferase Assay: HEK293 cells are transfected with GLI1 expression plasmid, together with the reporter plasmids 12×GliBSLuc and R-Luc on 10-cm plates (day 0). Twenty-four hours later, cells are seeded in white 96-well plates with clear bottom at a density of 15,000 cells per well. Cells are allowed to attach, and compounds are added at a final concentration of 10 μM in DMSO (0.5% final DMSO concentration) (day 1.5). Cells are grown for another 24 h, subsequently lysed, and then analyzed by using the Dual Luciferase kit.
Cell Research	BrdU Incorporation Assay. Subconfluent cells are grown in reduced FBS (2.5%) for 48 h in the presence of 5 μM test compound (or DMSO) on white 96-well plates with clear bottom. Subsequently, cells are labeled for 2 h with BrdU, fixed, and analyzed.(Only for Reference)

Synonyms	GANT61, NSC 136476
Molecular Weight	429.612
Formula	C27H35N5
CAS No.	500579-04-4

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 21.5 mg/mL (50 mM)

Ethanol: 43 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Lauth M, et al. Proc Natl Acad Sci, 2007, 104(20), 8455-8560 2. Desch P, et al. Oncogene, 2010, 29(35), 4885-4895. 3. Mazumdar Y, et al. Cancer Res, 2011, 71(3), 1092-1102. 4. Mazumdar T, et al. Cancer Res, 2011,71(17), 5904-5914. 5. Pan D, et al. Leuk Res, 2012, 36(6), 742-748. 6. Zheng H T, Fu T, Zhang H Y, et al. Progesterone-regulated Hsd11b2 as a barrier to balance mouse uterine corticosterone[J]. Journal of Endocrinology. 2020, 244(1): 177-187.

Citations

1. Zheng H T, Fu T, Zhang H Y, et al. Progesterone-regulated Hsd11b2 as a barrier to balance mouse uterine corticosterone. Journal of Endocrinology. 2020, 244(1): 177-187

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Autophagy Compound Library GPCR Compound Library Anti-Obesity Compound Library Anti-Pancreatic Cancer Compound Library Inhibitor Library Wnt/Hedgehog/Notch Compound Library Stem Cell Differentiation Compound Library Neuronal Signaling Compound Library Anti-Aging Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

GANT 61 500579-04-4 G蛋白偶联受体干细胞自噬 Autophagy Hedgehog/Smoothened GANT-61 NSC-136476 GANT61 NSC136476 Inhibitor NSC 136476 Gli inhibit inhibitor

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