Powder: -20°C for 3 years
In solvent: -80°C for 2 years
GANT 61 is an inhibitor for Gli1 and Gli2.
Description | GANT 61 is an inhibitor for Gli1 and Gli2. |
Targets&IC50 | GLI1:5 μM |
Kinase Assay | Dual Luciferase Assay: HEK293 cells are transfected with GLI1 expression plasmid, together with the reporter plasmids 12×GliBSLuc and R-Luc on 10-cm plates (day 0). Twenty-four hours later, cells are seeded in white 96-well plates with clear bottom at a density of 15,000 cells per well. Cells are allowed to attach, and compounds are added at a final concentration of 10 μM in DMSO (0.5% final DMSO concentration) (day 1.5). Cells are grown for another 24 h, subsequently lysed, and then analyzed by using the Dual Luciferase kit. |
Cell Research | BrdU Incorporation Assay. Subconfluent cells are grown in reduced FBS (2.5%) for 48 h in the presence of 5 μM test compound (or DMSO) on white 96-well plates with clear bottom. Subsequently, cells are labeled for 2 h with BrdU, fixed, and analyzed.(Only for Reference) |
Synonyms | GANT61, NSC 136476 |
Molecular Weight | 429.612 |
Formula | C27H35N5 |
CAS No. | 500579-04-4 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 21.5 mg/mL (50 mM)
Ethanol: 43 mg/mL (100 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
GANT 61 500579-04-4 G蛋白偶联受体 干细胞 自噬 Autophagy Hedgehog/Smoothened GANT-61 NSC-136476 GANT61 NSC136476 Inhibitor NSC 136476 Gli inhibit inhibitor