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FIPI

Catalog No. T3580 CAS 939055-18-2

Synonyms: 5-Fluoro-2-indolyl deschlorohalopemide

FIPI (5-Fluoro-2-indolyl deschlorohalopemide) is a derivative of halopemide which potently inhibits both PLD1 (IC50 = 25 nM) and PLD2 (IC50 = 20 nM); prevents PLD regulation of F-actin cytoskeleton reorganization, cell spreading, and chemotaxis.

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FIPI, CAS 939055-18-2

Pack Size	Availability	Price/USD
2 mg	In stock	$ 42.00
5 mg	In stock	$ 68.00
10 mg	In stock	$ 89.00
25 mg	In stock	$ 182.00
50 mg	In stock	$ 318.00
100 mg	In stock	$ 497.00
1 mL * 10 mM (in DMSO)	In stock	$ 72.00

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Purity: 99.08%

Purity: 98.01%

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Description	FIPI (5-Fluoro-2-indolyl deschlorohalopemide) is a derivative of halopemide which potently inhibits both PLD1 (IC50 = 25 nM) and PLD2 (IC50 = 20 nM); prevents PLD regulation of F-actin cytoskeleton reorganization, cell spreading, and chemotaxis.
Targets&IC50	PLD1:25 nM, PLD2:20 nM
In vitro	FIPI is a potent, concentration-dependent PLD2 inhibitor, and we show here that it inhibits PLD1 equally well under standard in vitro assay conditions. FIPI was added into the cell culture media 1 h before performing an in vivo PLD assay and was found to be a potent inhibitor of PLD2 with an IC50 of 10 nM.? The typical localization of PLD1 to peri-nuclear membrane vesicles and PLD2 to the plasma membrane were not affected by exposure to FIPI and and FIPI did not decrease PIP2 availability on the plasma membrane in PLD1- and PLD2-overexpressing cells as assessed using an enhanced GFP-fused PIP2 sensor. FIPI did not significantly inhibit p38 or ERK phosphorylation in bone marrow-derived macrophages stimulated with lipopolysaccharide. FIPI inhibition of PLD did diminish fMLP-directed chemotaxis(p < 0.01), validating this role for PLD function and suggesting that PLD regulates chemotaxis via mechanisms distinct from affecting MAKP signaling.
Kinase Assay	Phospholipase D activity is quantified using our established method of measuring the formation of [32P]-radiolabeled PBt. Cellular lipids are extracted and PBt is isolated using our published methods of lipid extraction and thin layer chromatographic separation, respectively. Radioactivity is measured using liquid scintillation counting and quantified as DPM normalized to 106 counts in the total cellular lipid extract or as percentage of control (vehicle-treated cells).
Cell Research	Cytotoxicity in MAECs is determined by assaying the extent of reduction in MTT in intact cells using the commercial MTT reduction assay kit. At the end of the experimental treatments, MTT solution (10% vol/vol in MEM) is added and the cells are incubated for 3 hours, following which MTT solvent is added in an amount equal to the original culture volume. Absorbance of the reduced MTT is determined spectrophotometrically, according to the manufacturer's recommendations.

Synonyms	5-Fluoro-2-indolyl deschlorohalopemide
Molecular Weight	421.47
Formula	C23H24FN5O2
CAS No.	939055-18-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 12.5 mg/mL (29.66 mM)

References and Literature

1. Monovich L, et al. Optimization of halopemide for phospholipase D2 inhibition. Bioorg Med Chem Lett. 2007 Apr 15;17(8):2310-1. 2. Su W, et al. 5-Fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol Pharmacol. 2009 Mar;75(3):437-46. 3. Secor JD, et al. Novel lipid-soluble thiol-redox antioxidant and heavy metal chelator, N,N'-bis(2-mercaptoethyl)isophthalamide (NBMI) and phospholipase D-specific inhibitor, 5-fluoro-2-indolyl des-chlorohalopemide (FIPI) attenuate mercury-induced lipid si 4. Li J, Yu F, Guo H, et al. Crystal structure of plant PLDα1 reveals catalytic and regulatory mechanisms of eukaryotic phospholipase D[J]. Cell Research. 2020, 30(1): 61-69.

Citations

1. Li J, Yu F, Guo H, et al. Crystal structure of plant PLDα1 reveals catalytic and regulatory mechanisms of eukaryotic phospholipase D. Cell Research. 2020, 30(1): 61-69. 2. Liu Z, Nan Y, Luo Q, et al. DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma. Cancer Research. 2022

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Lipid Metabolism Compound Library Bioactive Compounds Library Max Bioactive Lipid Compound Library NO PAINS Compound Library Immunology/Inflammation Compound Library Bioactive Compound Library Metabolism Compound Library Anti-Obesity Compound Library Fluorochemical Library

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Keywords

FIPI 939055-18-2 Autophagy Metabolism Phospholipase Inhibitor inhibit 5-Fluoro-2-indolyl deschlorohalopemide inhibitor

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