This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
DiR iodide
Catalog No. TD0085 CAS
TD0085
Synonyms:
DiR iodide [1,1‘-dioctadecyl-3,3,3’,3‘-tetramethylindotricarbocyanine iodide]
DiR iodide (DiR iodide [1,1‘-dioctadecyl-3,3,3’,3‘-tetramethylindotricarbocyanine iodide]) is an important near infrared (NIR) fluorescent dye, which has been widely used for in vivo monitoring of cells.
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DiR iodide (DiR iodide [1,1‘-dioctadecyl-3,3,3’,3‘-tetramethylindotricarbocyanine iodide]) is an important near infrared (NIR) fluorescent dye, which has been widely used for in vivo monitoring of cells.
In vitro
Stain adherent cells: Grow adherent cells on sterile glass coverslips. Remove coverslips from growth medium and gently drain off excess medium. Place coverslip in a humidity chamber. Pipet 100 μL of the dye working solution onto the corner of a coverslip and gently agitate until all cells are covered. Incubate the coverslip at 37℃ for 2-20 minutes. Drain off the dye working solution and wash the coverslips two to three times with pre-warmed growth medium. Microscopy Detection: DiI, 549/565 nm, XF32-Omega, 31002-Chroma; DiR, 748/780 nm, XF112-Omega, 41009-Chroma
Synonyms
DiR iodide [1,1‘-dioctadecyl-3,3,3’,3‘-tetramethylindotricarbocyanine iodide]
Molecular Weight
1013.39
Formula
C63H101IN2
CAS No.
TD0085
Storage
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.