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CM-H2DCFDA

Catalog No. T30991 CAS 1219794-09-8

Synonyms: CMH2DCFDA, CM H2DCFDA

CM-H2DCFDA is a fluorescent indicator for reactive oxygen species (ROS) in cells.

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CM-H2DCFDA, CAS 1219794-09-8

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

CM-H2DCFDA is a fluorescent indicator for reactive oxygen species (ROS) in cells.

Synonyms	CMH2DCFDA, CM H2DCFDA
Molecular Weight	577.79
Formula	C27H19Cl3O8
CAS No.	1219794-09-8

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Oparka M, Walczak J, Malinska D, van Oppen LMPE, Szczepanowska J, Koopman WJH, Wieckowski MR. Quantifying ROS levels using CM-H(2)DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11. doi: 10.1016/j.ymeth.2016.06.008. Epub 2016 Jun 11. Review. PubMed PMID: 27302663.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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The answer of 19.713 mg appears in the Mass box

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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

CM-H2DCFDA 1219794-09-8 CM-H-2DCFDA CMH2DCFDA CM H2DCFDA inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.