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B-AP15

Catalog No. T1932   CAS 1009817-63-3
Synonyms: NSC 687852

B-AP15 (NSC-687852)(NSC-687852) is a selective inhibitor of the deubiquitinating enzymes Usp14 and UCHL5 of the 26S proteasome. It blocks the deubiquitinating activity of the 26S proteasome.

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B-AP15 Chemical Structure
B-AP15, CAS 1009817-63-3
Pack Size Availability Price/USD Quantity
5 mg In stock $ 32.00
10 mg In stock $ 52.00
25 mg In stock $ 89.00
50 mg In stock $ 133.00
100 mg In stock $ 225.00
200 mg In stock $ 397.00
500 mg In stock $ 676.00
1 mL * 10 mM (in DMSO) In stock $ 44.00
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Purity: 98%
Purity: 95.25%
Purity: 95.07%
Purity: 91.43%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description B-AP15 (NSC-687852)(NSC-687852) is a selective inhibitor of the deubiquitinating enzymes Usp14 and UCHL5 of the 26S proteasome. It blocks the deubiquitinating activity of the 26S proteasome.
Targets&IC50 UCHL5:2.1 μM
Kinase Assay For deubiquitinase inhibition assays, 19S regulatory particle (5 nM), 26S (5 nM) UCH-L1 (5 nM), UCH-L3 (0.3 nM), USP2CD (5 nM) USP7CD (5 nM) USP8CD (5 nM) or BAP1 (5 nM) is incubated with DMSO or b-AP15 and monitored the cleavage of ubiquitin-AMC (1,000 nM) using a Wallac VICTOR Multilabel counter or a Tecan Infinite M1000 equipped with 380 nm excitation and 460 nm emission filters[1].
Cell Research b-AP15 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. Cell viability is monitored by either the fluorometric microculture cytotoxicity assay or the MTT assay. For the MTT assay, cells are seeded into 96-well flat-bottomed plates overnight and exposed to drugs, using DMSO as the control. At the end of incubations, 10 μl of a stock solution of 5 mg/mL MTT is added into each well, and the plates are incubated 4 hours at 37°C. Formazan crystals are dissolved with 100 μL 10% SDS/10 mM HCl solution overnight at 37°C. Absorbance is measured using an enzyme-linked immunosorbent assay (ELISA) plate reader at 590 nm[2].
Synonyms NSC 687852
Molecular Weight 419.39
Formula C22H17N3O6
CAS No. 1009817-63-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 41.9 mg/mL (100 mM)

TargetMolReferences and Literature

1. D'Arcy P, et al. Nat Med. 2011, 17(12):1636-40. 2. D'Arcy P, et al. Int J Biochem Cell Biol. 2012, 44(11):1729-38. 3. Lopez-Castejon G, et al. J Biol Chem, 2013, 288(4), 2721-2733.

TargetMolCitations

1. Wu W, Xu H, Liao C, et al. Blockade of USP14 potentiates type I interferon signaling and radiation-induced antitumor immunity via preventing IRF3 deubiquitination. Cellular Oncology. 2022: 1-15 2. Yue X, Liu T, Wang X, et al.Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1–BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy.Acta Pharmaceutica Sinica B.2023

Related compound libraries

This product is contained In the following compound libraries:
HIF-1 Signaling Pathway Compound Library Inhibitor Library Cysteine Covalent Library Anti-Aging Compound Library Covalent Inhibitor Library Ubiquitination Compound Library Cell Cycle Compound Library Bioactive Compound Library Apoptosis Compound Library Bioactive Compounds Library Max

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GNE-6776 XL177A P22074 USP5-IN-1 DUB-IN-3 HBX 41108 USP8-IN-3 KSQ-4279

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Keywords

B-AP15 1009817-63-3 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB Deubiquitinase Inhibitor BAP15 inhibit NSC-687852 DUBs B AP15 NSC 687852 b-AP15 B-AP-15 NSC687852 inhibitor

 

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