Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Apcin is a potent and competitive inhibitor of anaphase-promoting complex/cyclosome (APC/C(Cdc20)) E3 ligase activity. Apcin competitively inhibits APC/C-dependent ubiquitylation by binding to Cdc20 and preventing substrate recognition. It acts by blocking mitotic exit and being synergistically amplified by co-addition of Ts-Arg-OMe.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 40.00 | |
2 mg | In stock | $ 57.00 | |
5 mg | In stock | $ 89.00 | |
10 mg | In stock | $ 143.00 | |
25 mg | In stock | $ 288.00 | |
50 mg | In stock | $ 497.00 | |
100 mg | In stock | $ 748.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 98.00 |
Description | Apcin is a potent and competitive inhibitor of anaphase-promoting complex/cyclosome (APC/C(Cdc20)) E3 ligase activity. Apcin competitively inhibits APC/C-dependent ubiquitylation by binding to Cdc20 and preventing substrate recognition. It acts by blocking mitotic exit and being synergistically amplified by co-addition of Ts-Arg-OMe. |
In vitro | apcin as a small molecule ligand of Cdc20 that inhibits APC/CCdc20 and prolongs mitosis.?apcin paradoxically shortens mitosis when SAC activity is high.?These opposing effects of apcin arise from targeting of a common binding site in Cdc20 required for both substrate ubiquitination and MCC-dependent APC/C inhibition.?Furthermore, apcin cooperates with p31comet to relieve MCC-dependent inhibition of APC/C.?Apcin therefore causes either net APC/C inhibition, prolonging mitosis when SAC activity is low, or net APC/C activation, shortening mitosis when SAC activity is high, demonstrating that a small molecule can produce opposing biological effects depending on regulatory context. |
Molecular Weight | 438.65 |
Formula | C13H14Cl3N7O4 |
CAS No. | 300815-04-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 240 mg/mL (547.13 mM), sonification is recommended.
You can also refer to dose conversion for different animals. More
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Apcin 300815-04-7 Cell Cycle/Checkpoint APC ligase pocket anaphase-promoting recognition complex Inhibitor Cdc20 Anaphase promoting complex cyclosome ubiquitylation inhibit mitosis WD40-domain APC/C inhibitor