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Amifostine thiol

Catalog No. T37538 CAS 31098-42-7

Amifostine thiol (WR-1065), the active metabolite of cytoprotector Amifostine, serves as a cytoprotective agent possessing radioprotective properties. It activates p53 via a JNK-dependent signaling pathway[1][2][3].

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Amifostine thiol, CAS 31098-42-7

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2 mg	5 days	$ 58.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

Amifostine thiol mitigates both immediate and delayed adverse effects of radiation exposure across a range of linear energy transfer (LET) levels, including DNA damage and G1-phase arrest[1]. It provides protection to RKO36 cells against chromosomal damage and death caused by ionizing radiation, also safeguarding these cells from delayed genomic instability when administered at a concentration of 4 mM for 30 minutes[1]. While cytotoxic to RKO36 cells at millimolar concentrations, particularly with continuous exposure[1], at 40 μM, it effectively prevents delayed genomic instability, albeit without guarding against immediate chromosomal damage or cell death[1]. Additionally, amifostine thiol activates JNK, which leads to p53 phosphorylation at threonine 81[2], and alters phosphorylation of topoisomerase IIα, influencing its activity and affecting cell cycle progression in CHO AA8 cells[3].

Molecular Weight	134.24
Formula	C5H14N2S
CAS No.	31098-42-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 250 mg/mL (1862.34 mM)

DMSO: < 1 mg/mL (insoluble or slightly Soluble)

References and Literature

1. Jaroslaw Dziegielewski, et al. WR-1065, the Active Metabolite of Amifostine, Mitigates Radiation-Induced Delayed Genomic Instability. Free Radic Biol Med. 2008 Dec 15; 45(12): 1674-1681. 2. Olivier Pluquet, et al. The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J Biol Chem. 2003 Apr 4;278(14):11879-87. J Biol Chem. 2003 Apr 4;278(14):11879-87. 3. J. S. Murley, et al. WR-1065, an active metabolite of the cytoprotector amifostine, affects phosphorylation of topoisomerase IIα leading to changes in enzyme activity and cell cycle progression in CHO AA8 cells. Cell Prolif. 1997 Jun; 30(6): 283-294.

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Keywords

Amifostine thiol 31098-42-7 inhibitor inhibit

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