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8pyDTZ

Catalog No. T19167 CAS 2351898-91-2

8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission and improved water solubility. It can be used for in vivo luminescence imaging.

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8pyDTZ, CAS 2351898-91-2

Pack Size	Availability	Price/USD
25 mg	6-8 weeks	$ 1,330.00
50 mg	6-8 weeks	$ 1,740.00
100 mg	6-8 weeks	$ 2,500.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission and improved water solubility. It can be used for in vivo luminescence imaging.
In vitro	8pyDTZ, with a solubility of 1711 μM, significantly increases water solubility by 13-fold over DTZ (131 μM). This compound not only shows a capability for red-shifted chemiluminescence and bioluminescence emission compared to DTZ but also produces the most red-shifted emission, allowing for improved tissue penetration. In evaluations using human embryonic kidney 293T cells expressing luciferase (HEK 293T cells), the LumiLuc-8pyDTZ combination yields approximately 3-5 times more bioluminescence than the teLuc-8pyDTZ pair. Additionally, when comparing far-red emission intensities with a 600-700 nm bandpass filter, LumiLuc-8pyDTZ consistently achieves 1.6-3.9 times higher photon flux than teLuc-DTZ across substrate concentrations ranging from 6.25 to 100 μM, without affecting ATP levels in LumiLuc-expressing cells treated with 8pyDTZ.
In vivo	The LumiLuc-8pyDTZ pair was evaluated alongside various standard reporters within a tumor xenograft mouse model, exhibiting high sensitivity and eliminating the need for organic cosolvents for in vivo administration. Additionally, LumiScarlet-8pyDTZ demonstrated equivalence to Akaluc-AkaLumine, the most luminous ATP-dependent luciferase-luciferin pair, in detecting cells within deep mouse tissues.

Molecular Weight	378.43
Formula	C24H18N4O
CAS No.	2351898-91-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

EtOH+HCl: 2 mg/mL (5.28 mM), when pH is adjusted to 2. Sonication is recommended. (DMSO inactivates the activity of 8pyDTZ.)

References and Literature

1. Yeh HW, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

8pyDTZ 2351898-91-2 Others inhibitor inhibit

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