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5-BDBD

Name: 5-BDBD
Brand: TargetMol
SKU: T22518
Price: 36 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T22518Cas No. 768404-03-1

5-BDBD is a potent and selective P2X4 receptor antagonist. 5-BDBD inhibits rP2X4R-mediated currents with an IC50 of 0.75 μM. 5-BDBD completely blocked the basal and acute hyperalgesia induced by NTG.

5-BDBD

🥰Excellent

Purity: 97.78%

Catalog No. T22518Cas No. 768404-03-1

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$36	In Stock	In Stock
2 mg	$52	In Stock	In Stock
5 mg	$84	In Stock	In Stock
10 mg	$147	In Stock	In Stock
25 mg	$322	In Stock	In Stock
50 mg	$572	In Stock	In Stock
100 mg	$815	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$94	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.78%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	5-BDBD is a potent and selective P2X4 receptor antagonist. 5-BDBD inhibits rP2X4R-mediated currents with an IC50 of 0.75 μM. 5-BDBD completely blocked the basal and acute hyperalgesia induced by NTG.
Targets&IC50	P2X4:0.75 μM
In vitro	5-BDBD could be specifically used to discriminate between P2X1R, P2X2aR, P2X2bR, P2X3R, P2X4R, and P2X7R[1]. 5-BDBD inhibits 10 μM ATP-induced currents of rP2X4R-expressing HEK293 cells in a concentration-dependent manner, with an IC50 of 0.75 μM[1]. 5-BDBD displaces rightward the ATP concentration-response curve, with an EC50 of 4.7 to 15.9 μM[1].
In vivo	Basal hyperalgesia induced by recurrent NTG injection was completely blocked by 5-BDBD[2].
Animal Research	5-BDBD (28 mg/kg; i.p.; daily for 9 days; male C57BL/6 mice) prevented NTG-induced mechanical hypersensitivity[2].

Chemical Properties

Molecular Weight	355.19
Formula	C₁₇H₁₁BrN₂O₂
Cas No.	768404-03-1
Smiles	Brc1cccc(c1)C1=NCC(=O)Nc2c1oc1ccccc21
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (168.92 mM), Sonication and heating to 60℃ are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.63 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8154 mL	14.0770 mL	28.1539 mL	140.7697 mL
5 mM	0.5631 mL	2.8154 mL	5.6308 mL	28.1539 mL
10 mM	0.2815 mL	1.4077 mL	2.8154 mL	14.0770 mL
20 mM	0.1408 mL	0.7038 mL	1.4077 mL	7.0385 mL
50 mM	0.0563 mL	0.2815 mL	0.5631 mL	2.8154 mL
100 mM	0.0282 mL	0.1408 mL	0.2815 mL	1.4077 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc