Powder: -20°C for 3 years | In solvent: -80°C for 1 year
4-Aminohippuric Acid is the glycine amide of 4-aminobenzoic acid. Its sodium salt is used as a diagnostic aid to measure effective renal plasma flow (ERPF) and excretory capacity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 35.00 | |
100 mg | In stock | $ 47.00 | |
200 mg | In stock | $ 68.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | 4-Aminohippuric Acid is the glycine amide of 4-aminobenzoic acid. Its sodium salt is used as a diagnostic aid to measure effective renal plasma flow (ERPF) and excretory capacity. |
In vitro | In normal individuals, the renal extraction ratio of 4-Aminohippuric acid is approximately 0.92. After extraction, the majority of 4-Aminohippuric acid is actively secreted by the proximal tubules and completely excreted by the kidneys. The clearance of 4-Aminohippuric acid is utilized to measure renal plasma flow and to assess the secretory mechanisms or transport limits of renal tubules. The kinetics of 4-Aminohippuric acid uptake by cortical tubular cells have been measured using the stopped-flow microperfusion technique in rat kidneys. In a rat model of renal failure, intravenous injection of 3.0 mg/kg Uranyl nitrate significantly reduced the CLPAH to 0.89 mL/min/kg, compared to 29.30 mL/min/kg in normal rats. Rats with chronic kidney failure exhibited altered pharmacokinetics, showing decreased renal excretion of 4-Aminohippuric acid. Compared to Sham rats, those with chronic kidney failure demonstrated a weakened load of excretion, filtration, and secretion of 4-Aminohippuric acid, with about a 40% reduction in the expression of OAT1 in the homogenate and membrane. Hence, measuring the renal clearance of 4-Aminohippuric acid typically reflects the kidney's capacity for organic anion transport. |
In vivo | At a concentration of 10 mmol/L, 4-Aminohippuric acid was transported across membranes of HEK293 cells expressing MRP2 with a rate of 21.9 pmol/mg protein/min. The kinetic parameters for 4-Aminohippuric acid transport included a Km value of 880 mM and a Vmax value of 2.3 nmol/mg protein/min, indicating facilitated transport through organic anion transporters expressed in epithelial cells. Additionally, 4-Aminohippuric acid uptake by HEK293 cells, mediated by overexpressed and transfected NPT1, exhibited saturable kinetics in line with Michaelis-Menten dynamics, having a Km close to 2.66 mM and a Vmax of 940 pmol/mg protein/30s. This compound is also transported by transporters other than OAT, including NPT1 and MRP2. |
Synonyms | p-Aminohippuric acid |
Molecular Weight | 194.19 |
Formula | C9H10N2O3 |
CAS No. | 61-78-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 37 mg/mL (190.5 mM)
Ethanol: 2 mg/mL (10.29 mM)
H2O: 3 mg/mL (15.44 mM)
You can also refer to dose conversion for different animals. More
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4-Aminohippuric Acid 61-78-9 Metabolism Endogenous Metabolite Amino Acids and Derivatives Inhibitor inhibit 4Aminohippuric Acid p-Aminohippuric acid 4 Aminohippuric Acid 4-Aminohippuric acid inhibitor