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3-Bromo-7-nitroindazole

Name: 3-Bromo-7-nitroindazole
Brand: TargetMol
SKU: T10107
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10107Cas No. 74209-34-0

3-Bromo-7-nitroindazole is a specific neuronal nitric oxide synthase (nNOS) inhibitor that affects the synthesis of the intercellular messenger NO throughout the brain and body.

3-Bromo-7-nitroindazole

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Purity: 98.2%

Catalog No. T10107Cas No. 74209-34-0

3-Bromo-7-nitroindazole is a specific neuronal nitric oxide synthase (nNOS) inhibitor that affects the synthesis of the intercellular messenger NO throughout the brain and body.

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$46	In Stock	In Stock
100 mg	$76	In Stock	In Stock
500 mg	$189	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:98.2%

Appearance:Solid

Color:Yellow

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COA LCMS

Product Introduction

Bioactivity

Description

3-Bromo-7-nitroindazole is a specific neuronal nitric oxide synthase (nNOS) inhibitor that affects the synthesis of the intercellular messenger NO throughout the brain and body.

In vivo

Male rats received either 3-Bromo-7-nitroindazole (20mg/kg/day) or saline via intraperitoneal injection for 5days. Daily administration of the specific neuronal nitric oxide synthase (nNOS) inhibitor, 3-Bromo-7-nitroindazole impaired the acquisition of the Morris water maze (MWM) paradigm task. 3-Bromo-7-nitroindazole also impaired the probe trial. The MWM training was associated with a significant increase in the brain-derived neurotrophic factor (BDNF) mRNA expression in the hippocampus. BDNF mRNA expression in the hippocampus did not change after 3-Bromo-7-nitroindazole treatment. L-arginine significantly reversed behavioural parameters, and the effect of 3-Bromo-7-nitroindazole was found to be NO-dependent. There were no differences in locomotor activity and blood pressure in 3-Bromo-7-nitroindazole treated rats[1].

Chemical Properties

Molecular Weight	242.03
Formula	C₇H₄BrN₃O₂
Cas No.	74209-34-0
Smiles	[O-][N+](=O)C1=CC=CC2=C(Br)NN=C12
Relative Density.	1.8856 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (413.17 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (16.53 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1317 mL	20.6586 mL	41.3172 mL	206.5860 mL
5 mM	0.8263 mL	4.1317 mL	8.2634 mL	41.3172 mL
10 mM	0.4132 mL	2.0659 mL	4.1317 mL	20.6586 mL
20 mM	0.2066 mL	1.0329 mL	2.0659 mL	10.3293 mL
50 mM	0.0826 mL	0.4132 mL	0.8263 mL	4.1317 mL
100 mM	0.0413 mL	0.2066 mL	0.4132 mL	2.0659 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc