28-Deoxonimbolide

Name: 28-Deoxonimbolide
Brand: TargetMol
SKU: TN2835
Rating: 4.5 (1 reviews)

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28-Deoxonimbolide is a nimbolide-type limonoid natural product isolated from the seeds of Azadirachta indica. In vitro, it induces apoptosis in tumor cells (e.g., HL-60), with mechanisms involving mitochondrial pathways and potentially the participation of death receptor signaling. It is commonly used in studies of anticancer mechanisms.

Cas No. 126005-94-5

Pack Size	Price	USA Stock	Global Stock	Quantity
5 mg	$1,698	Inquiry	Inquiry

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	28-Deoxonimbolide is a nimbolide-type limonoid natural product isolated from the seeds of Azadirachta indica. In vitro, it induces apoptosis in tumor cells (e.g., HL-60), with mechanisms involving mitochondrial pathways and potentially the participation of death receptor signaling. It is commonly used in studies of anticancer mechanisms.
Targets & IC50	A549 cells:9.3 μM, SKBR3 cells:1.7 μM, SK-MEL-2 cells:2.05 μg/mL (ED50), P388 leukemia cells:0.66 μg/mL(ED50), HL-60 cells:2.7 μM, Col2:1.81 μg/mL(ED50), HT1080 cells:1.04 μg/mL(ED50), KB cells:1.3 μg/mL (ED50)
In vitro	28-Deoxonimbolide exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50 values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively[1]. 28-Deoxonimbolide induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control)[1]. 28-Deoxonimbolide diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3[1].

Chemical Properties

Molecular Weight	452.55
Formula	C₂₇H₃₂O₆
Cas No.	126005-94-5
Smiles	C[C@@]12[C@@]([C@]3([C@@]4([C@@](C)([C@H]1CC(OC)=O)C(=O)C=C[C@@]4(C)CO3)[H])[H])(O[C@]5(C2=C(C)[C@@H](C5)C=6C=COC6)[H])[H]
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

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Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

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10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

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Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

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