(-)-Agelastatin A is a member of the pyrrole-aminoimidazole marine alkaloid (PAI) family, capable of inhibiting β-catenin, GSK-3β, and osteopontin (OPN), exhibiting antiproliferative activity against various cancers such as leukaemia and breast cancer, and possessing a certain ability to penetrate the central nervous system (CNS).

BT549 cells:278 nM, Daudi cells:20 nM, CA46 cells:187 nM, Jurkat cells:74 nM, HL-60 cells:138 nM, A549 cells:1.05 μM, CEM cells:20 nM, U-937 cells:67 nM, IMR90 cells:1.11 μM, HeLa cells:708 nM

Methods: Agelastatin A (0-2 μM, 48 h) was used to treat U-937, HeLa, A549, BT549 and IMR90 cells, and the IC50 values at 48 hours were determined by MTS.
Results: The IC50 values of U-937, HeLa, A549, BT549 and IMR90 cells were 67 nM, 708 nM, 1.05 μM, 278 nM and 1.11 μM, respectively.
Methods: CEM, Jurkat, Daudi, HL-60 and CA46 cells were treated with Agelastatin A (0-1 μM, 48 h). The IC50 values at 48 h were determined by MTS assay.
Results: The IC50 values of CEM, Jurkat, Daudi, HL-60 and CA46 cells were 20, 74, 20, 138 and 187 nM, respectively. [1]