Powder: -20°C for 3 years | In solvent: -80°C for 1 year
5(6)-EET is a fully racemic version of the enantiomeric forms biosynthesized from arachidonic acid by cytochrome P450 enzymes. In solution, 5(6)-EET degrades into 5,6-DiHET and 5(6)-δ-lactone, which can be converted to 5(6)-DiHET and quantified by GC-MS. In neuroendocrine cells, such as the anterior pituitary and pancreatic islets, 5(6)-EET has been implicated in the mobilization of calcium and hormone secretion. 5(6)-EET is an inhibitor of T-type voltage-gated calcium channels (Cav3) that inhibits isoforms Cav3.1, Cav3.2 (IC50 = 0.54 μM), and Cav3.3 and decreases nifedipine-resistant phenylephrine-induced vasoconstriction in isolated mouse mesenteric arteries via Cav3.2 blockade when used at a concentration of 3 μM. In addition, it is a substrate of COX-1 and COX-2, as measured by oxygen consumption and product formation assays when used at a concentration of 50 μM. (±)5(6)-EET is provided as a mixture of the free acid and lactone.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
25 μg | 35 days | $ 113.00 | |
50 μg | 35 days | $ 198.00 | |
100 μg | 35 days | $ 353.00 | |
500 μg | 35 days | $ 1,090.00 |
Description | 5(6)-EET is a fully racemic version of the enantiomeric forms biosynthesized from arachidonic acid by cytochrome P450 enzymes. In solution, 5(6)-EET degrades into 5,6-DiHET and 5(6)-δ-lactone, which can be converted to 5(6)-DiHET and quantified by GC-MS. In neuroendocrine cells, such as the anterior pituitary and pancreatic islets, 5(6)-EET has been implicated in the mobilization of calcium and hormone secretion. 5(6)-EET is an inhibitor of T-type voltage-gated calcium channels (Cav3) that inhibits isoforms Cav3.1, Cav3.2 (IC50 = 0.54 μM), and Cav3.3 and decreases nifedipine-resistant phenylephrine-induced vasoconstriction in isolated mouse mesenteric arteries via Cav3.2 blockade when used at a concentration of 3 μM. In addition, it is a substrate of COX-1 and COX-2, as measured by oxygen consumption and product formation assays when used at a concentration of 50 μM. (±)5(6)-EET is provided as a mixture of the free acid and lactone. |
Molecular Weight | 320.473 |
Formula | C20H32O3 |
CAS No. | 87173-80-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: >50 mg/mL (per Rao Maddipati)
Ethanol: >50 mg/mL (per Rao Maddipati)
DMF: >50 mg/mL (per Rao Maddipati)
PBS (pH 7.2): >1 mg/mL (from 13(S)-HODE)
You can also refer to dose conversion for different animals. More
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(±)5(6)-EET 87173-80-6 5(6)-EET (±)5(6) EET (±)5(6)EET inhibitor inhibit