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Tubulin polymerization-IN-6

Catalog No. T61532

Tubulin polymerization-IN-6 (compound 5f) is a potent inhibitor of tubulin polymerization, with an IC50 of 1.09 μM. It not only inhibits cell migration and tube formation but also has anti-angiogenic properties. Additionally, Tubulin polymerization-IN-6 has been found to effectively hinder tumor growth in HT29 xenograft Balb/c nude mice [1].

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Tubulin polymerization-IN-6 Chemical Structure

Tubulin polymerization-IN-6, CAS N/A

Pack Size	Availability	Price/USD
25 mg	10-14 weeks	$ 1,520.00
50 mg	10-14 weeks	$ 1,980.00
100 mg	10-14 weeks	$ 2,500.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

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Description	Tubulin polymerization-IN-6 (compound 5f) is a potent inhibitor of tubulin polymerization, with an IC50 of 1.09 μM. It not only inhibits cell migration and tube formation but also has anti-angiogenic properties. Additionally, Tubulin polymerization-IN-6 has been found to effectively hinder tumor growth in HT29 xenograft Balb/c nude mice [1].
In vitro	Tubulin polymerization-IN-6 (compound 5f), across various concentrations and periods, demonstrates significant anti-cancer activities, as evidenced by its broad-spectrum anti-proliferative effects against several cancer cell lines (MCF-7, MDA-MB-231, A549, Hela, and HT29), with notable efficacy even against drug-resistant strains (MCF-7/TxR and A549/TxR). This compound inhibits colony formation by tumor cells, disrupts cell migration, and impedes angiogenesis in HUVEC cells. It achieves these effects, in part, by competing with colchicine for binding, thereby inhibiting tubulin polymerization. Moreover, Tubulin polymerization-IN-6 induces cell cycle arrest and apoptosis in HT29 cells by modulating relevant proteins, decreasing mitochondrial membrane potential, and increasing reactive oxygen species (ROS) production. Detailed analyses, including Western Blot and Immunofluorescence, reveal dose-dependent impacts on tubulin dynamics and on the regulation of proteins crucial for cell cycle progression and apoptosis, thereby highlighting the compound's potential as a multi-faceted anti-cancer agent.
In vivo	Tubulin polymerization-IN-6, also referred to as compound 5f, demonstrated a dose-dependent inhibitory effect on tumor growth in HT29 xenograft Balb/c nude mice when administered intraperitoneally at dosages ranging from 0 to 10 mg/kg once every two days for three weeks, achieving a tumor weight reduction of 75.5% at the highest dosage. Additionally, a single intravenous dose of 10 mg/kg in SD rats revealed significantly improved pharmacokinetic properties, including an eight-fold increase in half-life and a two-fold enhancement in the area under the curve (AUC), indicating better systemic exposure and potential effectiveness of Tubulin polymerization-IN-6 against cancerous tumors.

Molecular Weight	375.37
Formula	C19H21NO7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Tubulin polymerization-IN-6 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.