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trans-Ned 19

Name: trans-Ned 19
Brand: TargetMol
SKU: T12205L
Price: 66 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12205L Copy Product Info

Purity: 99.87%

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trans-Ned 19 is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist and two-pore channel (TPC) blocker. It binds with high affinity to the NAADP receptor to inhibit NAADP-dependent lysosomal/endolysosomal calcium release without affecting IP3 or cADPR-mediated calcium signaling pathways. Functionally, trans-Ned 19 inhibits glucose-induced calcium oscillations in pancreatic islets and low histamine concentration-induced endothelium-dependent vasodilation, and reduces Ebola virus infection of host cells. In immunomodulation, NAADP signaling blockade by trans-Ned 19 promotes the conversion of mouse Th17 cells to regulatory T cells (Tregs) and shows protective effects in intestinal inflammation models.

Cas No. 1354235-96-3

Pack Size	Price	USA Stock	Global Stock
1 mg	$66	-	In Stock
5 mg	$158	-	In Stock
10 mg	$243	-	In Stock
25 mg	$522	-	In Stock
50 mg	$868	-	In Stock
1 mL x 10 mM (in DMSO)	$179	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.87%

Appearance:Solid

Color:Yellow

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	trans-Ned 19 is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist and two-pore channel (TPC) blocker. It binds with high affinity to the NAADP receptor to inhibit NAADP-dependent lysosomal/endolysosomal calcium release without affecting IP3 or cADPR-mediated calcium signaling pathways. Functionally, trans-Ned 19 inhibits glucose-induced calcium oscillations in pancreatic islets and low histamine concentration-induced endothelium-dependent vasodilation, and reduces Ebola virus infection of host cells. In immunomodulation, NAADP signaling blockade by trans-Ned 19 promotes the conversion of mouse Th17 cells to regulatory T cells (Tregs) and shows protective effects in intestinal inflammation models.
In vitro	trans-Ned 19 (25–100 µM; 1 h) inhibits T-cell receptor (TCR)-stimulated Ca²⁺ signaling, cell activation, and proliferation in primary immature CD4⁺ T cells from C57BL/6N mice [1]. trans-Ned 19 (25–50 µM; 10 min) inhibits norepinephrine (HY-13715)-induced Ca²⁺ elevation in rat aortic smooth muscle cells in a concentration-dependent manner [2]. trans-Ned 19 (100 μM; 30 min) significantly increased the rate of spontaneous acrosome reaction in the acrosome reaction assay of permeabilized mouse sperm [3].
In vivo	trans-Ned 19 (20 mg/kg; intraperitoneal injection; administered once 2 hours prior to the first injection of anti-CD3 mAb, followed by a second dose 48 hours later) reduced disease severity in a mouse model of anti-CD3 mAb-induced intestinal inflammation, as evidenced by reduced weight loss [1].

Chemical Properties

Molecular Weight	514.59
Formula	C₃₀H₃₁FN₄O₃
Cas No.	1354235-96-3
Smiles	C(O)(=O)[C@@H]1CC2=C([C@H](N1)C3=CC(CN4CCN(CC4)C5=C(F)C=CC=C5)=C(OC)C=C3)NC=6C2=CC=CC6
Relative Density.	1.316 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (155.46 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9433 mL	9.7165 mL	19.4329 mL	97.1647 mL
5 mM	0.3887 mL	1.9433 mL	3.8866 mL	19.4329 mL
10 mM	0.1943 mL	0.9716 mL	1.9433 mL	9.7165 mL
20 mM	0.0972 mL	0.4858 mL	0.9716 mL	4.8582 mL
50 mM	0.0389 mL	0.1943 mL	0.3887 mL	1.9433 mL
100 mM	0.0194 mL	0.0972 mL	0.1943 mL	0.9716 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc