This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
Tat-βsyn-degron TFA
Catalog No. T83726
Tat-βsyn-degron, an α-synuclein knockdown peptide, integrates three domains: the HIV-1 Tat plasma membrane transduction domain, an α-synuclein binding domain (βsyn) representing amino acids 36-45 of β-synuclein, and a proteasomal targeting domain (degron). It effectively binds to recombinant α-synuclein, demonstrating a significant reduction in α-synuclein levels in primary rat cortical neuron cultures—a result reversible by the proteasomal inhibitor MG132. In vivo experiments reveal that administrating Tat-βsyn-degron at 40 mg/kg reduces α-synuclein levels in the brains of M38 transgenic mice overexpressing human A53T mutant α-synuclein. It also diminishes phosphorylated α-synuclein levels in the substantia nigra and the pons, alongside neuroinflammation in mice exposed to α-synuclein preformed fibrils, simulating spreading synucleinopathy. Furthermore, at a dosage of 6 µmol/kg intraperitoneally, Tat-βsyn-degron ameliorates dopaminergic neuron loss and improves motor deficits in mice suffering from MPTP-induced Parkinson’s disease, underscoring its potential therapeutic benefit.
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Tat-βsyn-degron, an α-synuclein knockdown peptide, integrates three domains: the HIV-1 Tat plasma membrane transduction domain, an α-synuclein binding domain (βsyn) representing amino acids 36-45 of β-synuclein, and a proteasomal targeting domain (degron). It effectively binds to recombinant α-synuclein, demonstrating a significant reduction in α-synuclein levels in primary rat cortical neuron cultures—a result reversible by the proteasomal inhibitor MG132. In vivo experiments reveal that administrating Tat-βsyn-degron at 40 mg/kg reduces α-synuclein levels in the brains of M38 transgenic mice overexpressing human A53T mutant α-synuclein. It also diminishes phosphorylated α-synuclein levels in the substantia nigra and the pons, alongside neuroinflammation in mice exposed to α-synuclein preformed fibrils, simulating spreading synucleinopathy. Furthermore, at a dosage of 6 µmol/kg intraperitoneally, Tat-βsyn-degron ameliorates dopaminergic neuron loss and improves motor deficits in mice suffering from MPTP-induced Parkinson’s disease, underscoring its potential therapeutic benefit.
Molecular Weight
3146.67
Formula
C132H237N59O31.XCF3COOH
Storage
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMF: 50 mg/ml
PBS pH 7.2: 1 mg/ml
Dose Conversion
You can also refer to dose conversion for different animals.
More
Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculator
Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
bottom
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.