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T56-LIMKi

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Catalog No. T3960Cas No. 924473-59-6

Alias T5601640

T56-LIMKi (T5601640) is a selective inhibitor of LIMK2.

T56-LIMKi

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Purity: 99.57%

Catalog No. T3960Alias T5601640Cas No. 924473-59-6

T56-LIMKi (T5601640) is a selective inhibitor of LIMK2.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$40	In Stock	In Stock
10 mg	$68	In Stock	In Stock
25 mg	$139	In Stock	In Stock
50 mg	$222	In Stock	In Stock
100 mg	$347	-	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.57%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	T56-LIMKi (T5601640) is a selective inhibitor of LIMK2.
Targets&IC50	LIMK2:35.2 μM.
In vitro	T56-LIMKi effectively suppresses the proliferation of various cancer cell lines, including ST88-14, U87, Panc-1, and A549 lung cancer cells, exhibiting IC50 values of 18.3, 7.4, 35.2, and 90 µM respectively. It significantly lowers phosphorylated cofilin (p-cofilin) levels, impairing the growth of pancreatic, glioma, and schwannoma cancer cells. This compound inhibits cofilin phosphorylation, crucial for actin disruption, thereby preventing tumor cell movement, proliferation, and the formation of colonies in soft agar without contact. At concentrations ranging from 10-50 µM, T56-LIMKi dose-dependently decreases p-cofilin in NF1−/− MEFs, with a 30 µM IC50, while not affecting total cofilin levels. Furthermore, a 50 µM dosage of T56-LIMKi significantly reduces the presence of stress fibers in cells.
In vivo	T56-LIMKi induces inhibition of cofilin phosphorylation and promotes Panc-1 tumor shrinkage in vivo, with mice treated with T56-LIMKi (60 mg/kg) showing a significant decrease in tumor volume compared to controls[1].
Cell Research	T56-LIMKi can induce inhibition of cofilin phosphorylation and Panc-1 tumor shrinkage in vivo. Mice treated with T56-LIMKi (60 mg/kg) shows a significant decrease in tumor volume compared to control[1].
Synonyms	T5601640

Chemical Properties

Molecular Weight	389.33
Formula	C₁₉H₁₄F₃N₃O₃
Cas No.	924473-59-6
Smiles	Cc1cc(on1)C(=O)Nc1cccc(c1)C(=O)Nc1cccc(c1)C(F)(F)F
Relative Density.	1.426 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (128.43 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2.5 mg/mL (6.42 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5685 mL	12.8426 mL	25.6852 mL	128.4258 mL
5 mM	0.5137 mL	2.5685 mL	5.1370 mL	25.6852 mL
10 mM	0.2569 mL	1.2843 mL	2.5685 mL	12.8426 mL
20 mM	0.1284 mL	0.6421 mL	1.2843 mL	6.4213 mL
50 mM	0.0514 mL	0.2569 mL	0.5137 mL	2.5685 mL
100 mM	0.0257 mL	0.1284 mL	0.2569 mL	1.2843 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc